Bioprocess Eng., 15, 253-256 (1996)

Continuous extraction of a recombinant cutinase from Escherchia coli disrupted cells with reversed micelles using a perforated rotating disc contactor

Carneiro-da-Cunha, M. G., Aires-Barros, M. R., Tambourgi, E. B., Cabral, J. M. S.

Abstract

This work reports on the continuous extraction of a recombinant cutinase with reversed micelles using a perforated rotating disc contactor. Intracellular cutinase was directly extracted at 20°C with 100 mM AOT in isooctane from complex biological media of Escherichia coli disrupted cells. The optimal conditions for the direct extraction of the enzyme from media containing cell debris led to an extraction yield of 54.4%.

Keywords: -

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