J. Mol. Catal. B: Enzym., 1, 53-60 (1996)

Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites

Gonçalves, A. P. V., Lopes, J. M., Lemos, F., Ribeiro, F. R., Prazeres, D. M. F., Cabral, J. M. S., Aires-Barros, M. R.

Abstract

Fusarium solani pisi recombinant cutinase was immobilised by adsorption on different zeolites and its activity towards the hydrolysis reaction of tricaprylin was measured. It was observed that the enzyme immobilised over most of the zeolites used (NaA, NaX, NaY, NaUSY and modified forms) was active for this reaction, although the specific activity depended strongly on the structure and composition of the zeolite support. This preliminary study of the use of zeolites as supports for enzymes was also extended by the analysis of the influence of the water content and of the thermal stability of one of the preparations. It can be concluded that the prospective use of zeolites as supports for enzymes in reactions involving organic media is very promising.

Keywords: zeolites; lipase; hydrolysis

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