BioEngineering Research GroupBiocat. Biotrans., 25, 144-150 (2007)
de Carvalho, C. C. C. R., Marques, M. P. C., Fernandes, P., da Fonseca, M. M. R.
The degradation of alkanes and alcohols by Rhodococcus erythropolis DCL14 growing cells was tested at different reactor scales, ranging from a 2 L fermenter to 300 μL 96-well plates. The highest growth rates were attained in the fermenter, where pH, temperature and aeration rate in particular, are monitored and controlled. However, the results acquired in reactors smaller than 100 mL indicate that these reactors may be successfully used in testing carbon sources since the normalised results obtained with the different carbons sources were maintained in the 100-0.3 mL range. This study also shows the great potential of R. erythropolis DCL14 cells to degrade a wide range of alcohols and alkanes, which may be used in bioremediation of contaminated sites.
Keywords: Rhodococcus erythropolis; biodegradation and bioremediation; reactor scale; operational parameters
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