IEEE Sensors Lett., 7, 415-416 (2007)

Detection of chemiluminescence using an amorphous silicon photodiode

Pimentel, A. C, Pereira, A. T., Chu, V., Prazeres, D. M. F., Conde, J. P.

Abstract

A hydrogenated amorphous silicon photodiode is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm resulting from the horseradish peroxidase-catalyzed oxidation of luminol is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The sensitivity achieved is in the range of nanomole of horseradish peroxidase per liter.

Keywords: chemiluminescence; horseradish peroxidase; photodiode; protein detection

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