Bruno Alves
Master Student
Background
Graduation in Biotechnological Engineering, ULHT, 2010
Research Activities
Magnetic separations have gained interest over the past years as an alternative to standard purification methods for biomolecules. Given the non-magnetic behavior of most process contaminants, magnetic adsorbents can be highly selective and used for the direct processing of crude samples with suspended solid material. Therefore, by providing a capturing and primary purification step, process integration is possible. In the present work different coated magnetic particles will be used as adsorbents and assessed for their binding capacity and selectivity towards human immunoglobulin G (IgG). Ultimately, the feasibility of using the aforementioned magnetic adsorbents in the direct purification of IgG from a CHO cell culture supernatant will be evaluated.