Oliveira, P.H., Prather, K.L.J., Prazeres, D.M.F., Monteiro, G.A.,Mutation detection in plasmid-based biopharmaceuticals, Biotechnol. J., doi: 10.1002/biot.201000372 (2011)
Fernandes, P., Enzymes in food processing a condensed overview on strategies for better biocatalysts, Enzyme Res., doi:10.4061/2010/862537 (2010)
de Barros, D.P.C. , Azevedo, A. M., Cabral, J.M.S., Fonseca, L.P., Optimisation of flavour esters synthesis by Fusarium solani pisi cutinase, Journal of Food Biochemistry, xx, xx-xx (2010)
de Barros, D.P.C. , Fernandes, P., Cabral, J.M.S., Fonseca, L.P., Operational stability of cutinase in organic solvent system: model esterification of alkyl esters, J. Chem. Technol. Biotechnol., xx, xx-xx (2010) doi: 10.1002/jctb.2464
de Barros, D.P.C. , Lemos, F., Fonseca, L.P., Cabral, J.M.S., Kinetic cutinase-catalyzed esterification of caproic acid in organic solvent system, J. Mol. Catal. B.: Enzym, xx, xx-xx (2010) doi: 10.1016/j.molcatb.2010.06.005
de Barros, Dragana P.C., Fonseca, L.P., Cabral, J.M.S., Aschenbrenner, E.M., Weiss, C.K., Landfester, K., Miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters, Biotechnol. Bioeng., xx, xx-xx (2010) doi:10.1002/bit.22726
Botelho-Cunha, V.A., Mateus, M., Petrus, J.C.C., de Pinho, M.N., Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides, Biochem. Eng. J., 50, 29-36 (2010)
Carvalho, J., Azzoni, A.R., Prazeres, D.M.F., Monteiro, G.A., Comparative analysis of antigen targeting sequences used in DNA vaccines, Mol. Biotechnol., 44, 204-212 (2010) doi:10.1002/bit.22726
Carvalho, J., Rodgers, J., Atouguia, J., Prazeres, D.M.F., Monteiro, G.A., DNA vaccines: a rational design against parasitic diseases, Expert Rev. Vaccines, 9, 175-191 (2010) doi:10.1586/erv.09.158
de Carvalho, C.C.C.R., Enzymatic and whole cell catalysis: finding new strategies for old processes, Biotechnology Advances, x, xx-xx, (2010) DOI: 10.1016/j.biotechadv.2010.09.001
de Carvalho, C.C.C.R., Fernandes, P., Production of Metabolites as Bacterial Responses to the Marine Environment, Marine Drugs, 8 (3), 705-727 (2010)
Fernandes, P., Miniaturization in Biocatalysis, Int. J. Mol. Sci., 11, 858-879 (2010)
Gomes, A.G., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption, J. Chromatogr. A, 1217, 2262-2266 (2010)
Madeira, C., Mendes, R.D., Ribeiro, S.C., Boura, J.S, Aires-Barros, M.R., da Silva, C.L., Cabral, J.M.S., Non-Viral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy, J. Biomed. Biotechnol., xx, xx-xx(2010)
Marques, M.P.C., Cabral, J.M.S., Fernandes, P., A microwell platform for the scale-up of a multistep bioconversion to bench-scale reactors: Sitosterol side-chain cleavage, Biotechnol. J., (2010), doi:10.1002/biot.200900098
Marques, M.P.C., Cabral, J.M.S., Fernandes, P., Bioprocess scale-up: quest for the parameters to be used as criterion to move from microreactors to lab-scale, J. Chem. Technol. Biotechnol., (2010), 85, 11841198
Marques, M.P.C., Carvalho, F., de Carvalho, C.C.C.R., Cabral, J.M.S., Fernandes, P., Steroid bioconversion: Towards green processes?, , Food and Bioproducts Processing, 88 (1), 12-20 (2010)
Marques, M.P.C., de Carvalho, C.C.C.R., Cabral, J.M.S., Fernandes, P., Scaling-up of complex whole-cell bioconversions in conventional and non-conventional media, Biotechnol. Bioeng., (2010), 106, 619626
Marques, M.P.C., Fernandes, P., Cabral, J.M.S., Znidarsic-Plazl, P., Plazl, I., On the feasibility of in situ steroid biotransformation and product recovery in microchannels, Chem. Eng. J., (2010), 160, 708-714
Martins, S.A.M., Prazeres, D.M.F., Fonseca, L.P., Monteiro, G.A., Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification, Biotechnol. Lett., 32, 229-234 (2010) doi:10.1007/s10529-009-0149-
Oliveira, P.H., Prather, K.J., Prazeres, D.M.F., Monteiro, G.A., Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events, Appl. Microbiol. Biotechnol., xx, xx-xx (2010) doi:10.1007/s00253-010-2671-7
Raiado-Pereira, L., Prazeres, D.M.F., Mateus, M., Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization, J. Sep. Sci., 33, 1175-1184 (2010)
Ribeiro, S.C., Monteiro, G. A., Prazeres, D. M. F., Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin, Appl. Microbiol. Biotechnol., 86, 671-679 (2010) doi:10.1002/jps.21503
Ribeiro, S.C., Mendes, R., Madeira, C., da Silva, C.L., Cabral, J.M.S., A quantitative method to evaluate mesenchymal stem cell lipofection using real-time PCR. Biotechnology Progress, Biotechnol. Progr., xx, xx-xx (2010)
Rosa, P.A.J., Ferreira, I.F., Azevedo, A.M., Aires-Barros, M.R., Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals, J. Chromatogr. A, 1217 (16), 2296-2305 (2010)
Tyagi, M., da Fonseca, M.M.R., de Carvalho, C.C.C.R., Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes, Biodegradation, xx, xx-xx (2010), DOI: 10.1007/s10532-010-9394-4
Azevedo, A. M., Gomes, A. G., Rosa, P. A. J, Ferreira, I. F., Pisco, A. M. M. O., Aires-Barros, M. R., Partitioning of human antibodies in polyethylene glycol - sodium citrate aqueous two-phase systems, Sep. Pur. Tecnhol., 65, 14-21 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing, Trends Biotecnol., 27, 240-247 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Pisco, A. M. M. O., de Vries, J., Korporaal, R., Visser, T. J., Aires-Barros, M. R., Affinity-enhanced purification of human antibodies by aqueous two-phase extraction, Sep. Pur. Tecnhol., 65, 31-39 (2009) doi:10.1016/j.seppur.2008.03.006
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., de Vries, J., Visser, T. J., Aires-Barros, M. R., Downstream processing of human antibodies integrating an extraction capture step, cation exchange chromatography, J. Chromatogr. B, 887, 50-58 (2009) doi:10.1016/j.jchromb.2008.11.014
de Barros, D. P. C. , Fonseca, L. P., Cabral, J. M. S., Weiss, C. K., Landfester, K., Synthesis of alkyl esters by cutinase in miniemulsion and organic solvent media, Biotechnol. J., 4, 674-683 (2009) doi:10.1002/biot.200800294
de Barros, D. P. C. , Fonseca, L. P., Fernandes, P., Cabral, J. M. S., Mojovic L., Biosynthesis of ethyl caproate and other short ethyl esters catalyzed by cutinase in organic solvent, J. Mol. Catal. B.: Enzym., xx, xx-xx (2009) doi:10.1016/j.molcatb.2009.05.004
Cabeηa, R., Rodrigues, M., Prazeres, D. M. F., Chu, V., Conde, J. P., The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA, Nanotechnology, 20, 015503 (2009) doi:10.1088/0957-4484/20/1/015503
Cardoso, M. A. T., Antunes, S., van Keulen, F., Ferreira, B. S., Geraldes, A., Cabral, J. M. S., Palavra, A. M. F., SAS micronization of synthetic all trans-beta-carotene with tetrahydrofuran as solvent and carbon dioxide as antisolvent, J. Chem. Technol. Biotechnol., 84, 215-222 (2008) doi:10.1002/jctb.2027
Carvalho, C. C. C. R. de, Wick, L. Y., Heipieper, H. J., Cell wall adaptations of planktonic and biofilm Rhodococcus erythropolis cells to growth on C5 to C16 n-alkane hydrocarbons, Appl. Microbiol. Biotechnol., 82, 311320 (2009)
Carvalho, F., Marques, M. P. C., Carvalho C. C. C. R., Cabral, J. M. S., Fernandes, P., Sitosterol bioconversion with resting cells in liquid polymer based systems, Bioresource Technol., 100, 4050-4053 (2009) doi:10.1016/j.biortech.2009.03.044
Carvalho, J., Prazeres, D. M. F., Monteiro, G. A., Bringing DNA vaccines closer to commercial use, iDrugs, 12, 642-647 (2009)
Fernandes, P., Marques, M. P. C., Carvalho, F., Cabral, J. M. S., A simple method for biocatalyst immobilization using PVA-based hydrogel particles, J. Chem. Technol. Biotechnol., 84, 561-564 (2009) doi:10.1002/jctb.208
Freitas, S. S., Canαrio, S., Santos, J. A. L., Prazeres, D. M. F., Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact, Biotecnhol. J., 4, 265-278 (2009)
Freitas, S. S., Santos, J. A. L., Prazeres, D. M. F., Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase, Sep. Pur. Tecnhol., 65, 95-104 (2008) doi:10.1016/j.seppur.2008.04.001
Gomes, A. G., Azevedo, A. M., Aires-Barros, M. R., Prazeres, D. M. F., Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulphate, Sep. Pur. Tecnhol., 65, 22-30 (2009) doi:10.1016/j.seppur.2008.01.026
Guerrero-Germαn, P., Prazeres, D. M. F., Guzmαn, R., Montesinos-Cisneros, R. M., Tejeda-Mansir, A., Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography, Bioproces. Biosyst. Eng., 32, 615-622 (2009) doi:10.1007/s00449-008-0284-7
Henriques, A. M., Madeira, C., Fevereiro, M., Prazeres, D. M. F., Aires-Barros, M. R., Monteiro, G. A, Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi-Visna virus, Int. J. Pharm., 377, 92.98 (2009) doi:10.1016/j.ijpharm.2009.05.005
Marques, M. P. C., Cabral, J. M. S., Fernandes, P., High throughput in biotechnology: from shake-flasks to fully instrumented microfermentors, Recent Pat. Biotechnol., 3, 124-140 (2009)
Marques, M. P. C., Carvalho, F., Magalhγes, S., Cabral, J. M. S., Fernandes, P., Screening for suitable solvents as substrate carriers for the microbial side-chain cleavage of sitosterol using microtitre plates, Process Biochem., 44, 174-180 (2009) doi:10.1016/j.procbio.2009.01.014
Marques, M. P. C., Magalhγes, S., Cabral, J. M. S., Fernandes, P., Characterization of 24-well microtiter plate reactors for a complex multi-step bioconversion: from sitosterol to androstenedione, J. Biotechnol., 141, 556-561 (2009) doi:10.1016/j.jbiotec.2009.03.007
Martins, S. A. M., Prazeres, D. M. F., Fonseca, L. P., Monteiro, G. A., The role of probe-probe interactions on the hybridization of double stranded DNA targets onto DNA-modified magnetic microparticles, Anal. Bioanal. Chem., 394, 1711-1716 (2009) doi:10.1007/s00216-009-2843-4
Martins, S. A. M., Prazeres, D. M. F., Fonseca, L. P., Monteiro, G. A., Application of central composite design for DNA hybridization onto magnetic microparticles, Anal. Biochem., 391, 17-23 (2009) doi:10.1016/j.ab.2009.05.006
Oliveira, P. H., Prather, K. J., Prazeres, D. M. F., Monteiro, G. A., Structural instabilities of plasmid biopharmaceuticals: Challenges and implications, Trends Biotechnol, 27, 503-509 (2009) doi:10.1016/jtibtech.2009.06.004
Oliveira, P. H., Prazeres, D. M. F., Monteiro, G. A., Deletion-formation mutations in plasmid expression vectors are unfavoured by runaway amplification conditions and differentially selected under kanamycin stress, J. Biotechnol, 143, 231-238 (2009) doi:10.1016/j.jbiotec.2009.08.002
Paixγo, L., Rodrigues, L., Couto, I., Martins, M., Fernandes, P., de Carvalho, C.C.C.R., Monteiro, G.A., Sansonetty, F., Amaral, L., Viveiros, M., Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli, J. Biol. Eng., 3:18, 13 pages (2009) doi:10.1186/1754-1611-3-18
Pereira, A. T., Pimentel, A. C, Chu, V., Prazeres, D. M. F., Conde, J.P., Chemiluminescence detection of horseradish peroxidase using an integrated using an amorphous silicon thin film photosensor, IEEE Sensors, 9, 1282-1290 (2009)
Pimentel, A. C, Pereira, A. T., Prazeres, D. M. F., Chu, V., Conde, J. P., Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using and integrated amorphous silicon photodetector, Appl. Physics Lett., 94, 164106 (2009)
Pinotti, L. M., Fonseca, L. P., Prazeres, D. M. F., Rodrigues, D. S., Nuccia, E. R., Giordano, R. L. C., Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column, Biochem. Eng. J., 44, 111-118 (2009)
Ribeiro, S. C., Monteiro, G. A., Prazeres, D. M. F., Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies , J. Pharm. Sci., 98, 1400-1408 (2009) doi:10.1002/jps.21503
Rosa, P .A. J., Azevedo, A. M., Ferreira, I. F., Sommerfeld, S., Bδcker, W., Aires-Barros, M. R., Downstream processing of antibodies: Single-stage versus multi-stage aqueous two-phase extraction, J. Chromatogr. A, xx, xx-xx (2009) doi:10.1016/j.chroma.2009.02.024
Rosa, P. A. J., Azevedo A. M., Sommerfeld, S., Mutter, M., Aires-Barros, M. R., Bδcker, W., Application of aqueous two-phase systems to antibody purification: A multi-stage approach, J. Biotecnol., 139, 306-313 (2009)
Silva, A. I. , Mateus, M., Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies, J. Biotecnhol., 139, 236-249 (2009)
Silva, M. S., Prazeres, D. M. F., Lanηa, A., Atouguia, J., Monteiro, G. A., Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African Trypanosomiasis, Parasitol. Res., 105, 1223-1229 (2009) doi:10.1007/s00436-009-1542-6
Sousa, A., Sousa, F., Prazeres, D. M. F., Queiroz, J. A., Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention, Biomedical Chromatog., 23, 745-753 (2009) doi:10.1002/bmc.1179
Sousa, F., Prazeres, D. M. F., Queiroz, J. A., Improvement of transfection efficiency by using supercoiled plasmid DNA purified from a clarified lysate with arginine affinity chromatography, J. Gene Med., 11, 79-88 (2009) doi:10.1002/jgm.1272
Sousa, F., Prazeres, D .M. F., Queiroz, J. A., Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification, Biomedical Chromatog., 23, 160-165 (2008) doi:10.1002/bmc.1097
Sousa, I. T., Ruiu, L., Lowe, C. R., Taipa, M. A., Synthetic affinity ligands as a novel tool to improve protein stability, J. Mol. Recognit., 22, 83-90 (2009) doi:10.1002/jmr.900
Wu, M. L., Freitas, S. S., Monteiro, G. A., Prazeres, D. M. F., Santos, J. A. L., Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high shear vortices, Biotechnol. Appl. Biochem., 53, 237-246 (2009) doi:10.1042/BA20080215
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography, J. Chromatogr. A, 1213, 154-161 (2008)
Baptista, R. P., Pedersen, S., Cabrita, G. J., Otzen, D. E., Cabral, J. M. S., Melo E. P., Thermodynamics and mechanism of cutinase stabilization by trehalose, Biopolymers, 89, 538-547 (2008)
Brissos, V., Eggert, T., Cabral, J. M. S., Jaeger, K. E., Improving activity and stability of cutinase towards the anionic detergent AOT by complete saturation mutagenesis, Prot. Eng. Design Select., 21, 387-393 (2008)
Brissos, V., Melo, E. P., Martinho, J. M. G. , Cabral, J. M. S., Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT, Biochem. Biophys. Acta - Prot. Proteom., 1784, 1326-1334 (2008)
Cardoso, F. A., Germano, J., Ferreira, R., Cardoso, S., Martins, V. C., Freitas, P. P., Piedade, M. S., Sousa, L., Detection of 130 nm magnetic particles by a portable electronic platform using spin valve and magnetic tunnel junction sensors, J. Appl. Phys., 103, 07A310 (2008)
Cardoso, M. A. T, Cabral, J. M. S., Palavra, A. M. F., Geraldes, V., CFD analysis of supercritical antisolvent (SAS) micronization of minocycline hydrochloride, J. Supercritical Fluids, 47, 247-258 (2008)
Cardoso, M. A. T., Geraldes, V., Cabral, J. M. S., Palavra, A. M. F., Characterization of minocycline powder micronized by a supercritical antisolvent (SAS) process, J. Supercritical Fluids, 46, 71-76 (2008)
Carvalho, R. H., Lemos, M. A. N. D. A., Lemos, F., Cabral, J. M. S., Ribeiro, F. R., Electro-oxidation of phenol on zeolite/graphite composite electrodes - Part 3. Influence of the electrolyte and of nonelectroactive cations, Catal. Today, 133, 855-862 (2008)
Catarino, I., Minhalma, M., Beal, L. L., Mateus, M., de Pinho, M. N., Assessment of saccharide fractionation by ultrafiltration and nanofiltration, J. Memb. Sci., 312, 34-40 (2008) doi:10.1016/j.memsci.2007.12.057
Claudino, M. J. C., Soares, D., Marques, M. P. C., van Keulen, F., Cabral, J. M. S., Fernandes, P., Immobilization of mycobacterial cells onto silicone - assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol, Bioresource Technol., 99, 2304-2311 (2008)
Conde, J. P., Pimentel, A. C., Pereira, A. T., Gouvκa, A., Prazeres, D. M. F., Chu, V., Detection of molecular tags with an integrated amorphous silicon photodetector for biological applications, J. Non-Crystalline Sol., 354, 2594-2597 (2008)
Costa, L., Brissos, V., Lemos, F., Ribeiro, F. R., Cabral, J. M.S., Following multi-component reactions in liquid medium using spectral band-fitting techniques, Appl. Spectroscopy, 62, 932-935 (2008)
Costa, L., Brissos, V., Lemos, F., Ramτa Ribeiro, F., Cabral, J. M. S., Comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis, Bioprocess Biosystems Eng., 31, 323-327 (2008) doi:10.1007/s00449-007-0165-5
Costa, L. F. A., Lemos, F., Ramτa Ribeiro, F., Cabral, J. M. S., Zeolite screening for the racemization of 1-phenylethanol, Catal. Today, 133, 625-631 (2008) doi:10.1016/j.cattod.2007.11.041
Diogo, M. M., Henrique D., Cabral, J. M. S., Optimization and neural commitment of mouse embryonic stem cells, Biotechnol. Appl. Biochem., 49, 105-112 (2008)
Durγo, P., Chen, Z., Fernandes, A. T. Pereira, M. M., Melo, E. P., Martins, L. O., Copper incorporation into recombinant CotA-laccase from Bacillus subtilis: Characterization of Cu full-loaded enzymes, J. Biol. Inorg. Chem., 13, 183-193 (2008)
Fernandes, T. G., Kwon, S.-J., Lee, M.-Y., Clark, D. S., Cabral, J. M. S., Dordick, J. S., On-chip, cell-based microarray immunofluorescence assay for high-throughput analysis of target proteins, Anal. Chem., 80, 66336639 (2008)
Ferreira, I. F., Azevedo, A. M., Rosa, P. A. J., Aires-Barros, M. R., Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems, J. Chromatogr. A, 1195, 94-100 (2008)
Frias, A. M., Porada, C. D., Kirsten, B., Crapnell, C., Cabral, J. M. S., Zanjani, E.D., Almeida-Porada, G., Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion, Exp. Hematology, 36, 61-68 (2008)
Gonηalves, D., Prazeres, D. M. F., Chu, V., Conde, J. P., Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors, Biosens. Bioelectron., 24, 545-551 (2008)
Gonηalves, D., Prazeres, D. M. F., Chu, V., Conde, J. P., Amorphous silicon thin-film transistors gated through an electrolyte solution, IEEE Electron Device Lett., 29, 1030-1033 (2008)
Gouvκa, A., Pereira, A. T., Pimentel, A. C., Prazeres, D. M. F., Chu, V., Conde, J. P., Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate, Sensors Actuators B: Chemical, 135, 102-107 (2008) doi:10.1016/j.snb.2008.07.030
Lienqueo, M. E., Salazar, O., Calado, C. R. C., Fonseca, L. P., Cabral, J. M. S., Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography, Biotechnol. Lett., 30, 1353-1358 (2008)
Madeira, C., Loura, L. M. S., Prieto, M., Fedorov, A., M. Raquel Aires-Barros, M. R., Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET, BMC Biotechnol., 8:20 (2008)
Marques, M. P. C., Cabral, J. M. S., , Fernandes, P., Online oxygen monitoring system - non-conventional steroid fermentations in microtiter plates, Bioforum Europe, 9, 42-43 (2008)
Martins, S. A. M., Prazeres, D. M. F., Fonseca, L. P., Monteiro, G. A., Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples, Anal. Bioanal. Chem., 391, 2179-2187 (2008) doi:10.1007/s00216-008-2142-5
Oliveira, P. H., Lemos, F., Monteiro, G. A., Prazeres, D. M. F., Recombination frequency in plasmid DNA containing direct repeats - predictive correlation with repeat and intervening sequence length, Plasmid, 60, 159-165 (2008) doi:10.1016/j.plasmid.2008.06.004
Pimentel, A. C., Prazeres, D. M. F., Chu, V., Conde, J. P., Fluorescence detection of DNA using an amorphous silicon p-i-n photodiode, J. Appl. Phys., 104, 054913 (2008) doi:10.1063/1.2976343
Prazeres, D. M. F., Prediction of diffusion coefficients of plasmids, Biotechnol. Bioeng., 99, 1040-1044 (2008)
Ribeiro, S. C., Oliveira, P. H., Prazeres, D. M. F., Monteiro, G. A., High frequency plasmid recombination mediated by 28 bp direct repeats, Mol. Biotechnol., 40, 252-260 (2008) doi:10.1007/s12033-008-9082-3
Sampaio, P. N., Fortes, A. M., Cabral, J. M. S., Pais, M. S., Fonseca, L. P., Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain, J. Biosci. Bioeng., 105, 305-312, (2008)
Santos, A. M., Fedorov, A., Martinho, J. M. G., Baptista, R. P., Taipa, M. A., Cabral, J. M. S., Orientation of cutinase adsorbed onto PMMA nanoparticles probed by tryptophan fluorescence, J. Phys. Chem. B, 112, 3581-3585 (2008)
Sousa, F., Prazeres, D .M. F., Queiroz, J. A., Specific recognition of supercoiled plasmid DNA by arginine affinity chromatography, Anal. Biochem., 374, 432-434 (2008)
Sousa, F., Prazeres, D. M. F., Queiroz, J. A., Affinity chromatography approaches for overcoming the challenges of purifying plasmid DNA, Trends Biotechnol., 26, 518-525 (2008)
Taipa, M. A., Immunoassays: Biological tools for High Throughput Screening and characterisation of combinatorial libraries, CCHTS, 11, 325-355 (2008)
Teles, F. R. R., Fonseca, L.P., Trends in DNA biosensors, Talanta, 77, 606623 (2008)
Teles, F. R. R., Fonseca, L.P., Applications of polymers for biomolecule immobilization in electrochemical biosensors, Mat. Sci. Eng. C, 28, 15301543 (2008)
Tavares-Cardoso, M. A., Monteiro, G. A., Cardoso, J. P., Prazeres, T. J. V., Figueiredo, J. M. F., Martinho, J. M. G., Cabral, J. M. S., Palavra, A. M. F., Supercritical antisolvent micronization of minocycline hydrochloride, J. Supercritical Fluids, 44, 238-244 (2008) doi:10.1016/j.supflu.2007.09.035
Vale, G., Mota, A., Rial-Otero, R., Fonseca, L., Capelo, J. L., Ultrasonic-assisted enzymatic digestion (USAED) for total elemental determination and elemental speciation: A tutorial, Talanta, 75, 872-884, (2008)
Vale, G., Pereira, S., Mota, A., Fonseca, L., Capelo, J. L., Enzymatic probe sonication as a tool for solid-liquid extraction for total selenium determination by electrothermal-atomic absorption spectrometry, Talanta, 74, 198-205, (2008)
Vidinha, P., Augusto, V., Nunes, J., Lima, J. C., Cabral, J. M. S., Barreiros S. Probing the microenvironment of sol-gel entrapped cutinase: The role of added zeolite NaY, J. Biotechnol., 135, 181-189 (2008)
Vidinha, P., Barreiros, S., Cabral, J. M. S., Nunes, T. G., Fidalgo, A., Ilharco, L. M., Enhanced biocatalytic activity of ORMOSIL-encapsulated cutinase: The matrix structural perspective, J. Phys. Chem. C, 112, 2008-2015 (2008)
Vidinha, P., Lourenco, N. M. T., Pinheiro, C., Brαs, A. R., Carvalho, T., Santos-Silva, T., Mukhopadhyay, A., Romγo, M. J., Parola, J., Dionisio, M., Cabral, J. M. S., Afonso, C. A. M., Barreiros, S., Ion jelly: a tailor-made conducting material for smart electrochemical devices, Chem. Commun., 44, 5842-5844 (2008)
Vojinović, V., Cabral, J. M. S., Fonseca, L. P., Ex-situ bioprocess monitoring techniques, Chemical Industry & Chemical Engineering Quarterly, 13, 1-15, (2008)
Abranches, E., Bekman, E., Henrique, D., Cabral, J. M. S., Expansion of mouse embryonic stem cells on microcarriers, Biotechnol. Bioeng., 96, 1211-1221 (2007)
Azevedo, A. M. Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., Optimisation of aqueous two-phase extraction of human antibodies, J. Biotechnol., 132, 209-217 (2007)
Azzoni, A. R., Ribeiro, S. C., Monteiro, G. A., Prazeres, D. M. F., The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression, J. Gene Med., 9, 392-402 (2007)
Carapuηa, E., Azzoni, A. R., Prazeres, D. M. F., Monteiro, G. A., Mergulhγo, F. J. M., Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR, Mol. Biotechnol., 37, 120-126 (2007)
Carvalho, A. S. L., Ferreira, B. S., Neves-Petersen, M. T., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., Thermal denaturation of HRPA2: pH-dependent conformational changes, Enzyme Microb. Technol., 40, 696-703 (2007)
Carvalho, R. H., Lemos, F., Cabral, J. M. S., Ramτa Ribeiro, F., Influence of the presence of NaY zeolite on the activity of horseradish peroxidase in the oxidation of phenol, J. Mol. Catal. B.: Enzym., 44, 39-47 (2007)
Carvalho, R. H., Lemos, F., Lemos, M. A. N. D. A. Cabral, J. M. S., Ramτa Ribeiro, F., Electro-oxidation of phenol on a new type of zeolite/graphite biocomposite electrode with horseradish peroxidase, J. Mol. Catal. A, 278, 47-52 (2007)
de Carvalho, C. C. C. R., Marques, M. P. C., Fernandes, P., da Fonseca, M. M. R., Degradation of hydrocarbons and alcohols by Rhodococcus erythropolis DCL14: a comparison in scale performance, Biocat. Biotrans., 25, 144-150 (2007)
Catana, R., Eloy, M., Rocha, J. R., Ferreira, B. S., Cabral, J. M. S., Fernandes, P., Stability evaluation of an immobilized enzyme system for inulin hydrolysis, Food Chem., 101, 260-266 (2007)
Chen, Z., Franco, C. F., Baptista, R. P., Cabral, J. M. S., Coelho, A. V., Rodrigues Jr, C. J. Melo, E. P., Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides, Appl. Microbiol. Biotechnol., 73, 1306-1313 (2007)
Costa, M. J. L., Pedro, L., de Matos, A. P. A., Aires-Barros, M. R., Belo, J. A., Goncalves, J., Ferreira, G. N. M., Molecular construction of bionanoparticles: chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content, Biotechnol. Appl. Biochem., 48, 35-43 (2007)
Dias, A. I., Feliciano, A. S., Cabral, J. M. S., Prazeres, D. M. F., Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles, J. Colloid Int. Sci., 305, 198-201 (2007)
Duarte, D., Fortes, A. G., Prazeres, D. M .F., Marcos, J. C., Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process, J. Chromatog. A, 1164, 105-112(2007)
Fernandes, A. M., Fernandes, T. G., Diogo, M. M., Lobato da Silva C., Henrique D., Cabral, J. M. S., Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system, J. Biotechnol., 132, 227-236 (2007)
Fernandes, P., Cabral, J. M. S., Phytosterols: applications and recovery methods, Bioresource Technol., 98, 2325-2350 (2007)
Freitas, S. S., Azzoni, A. R., Santos, J. L., Monteiro, G. A., Prazeres, D. M. F., On the stability of plasmid DNA vectors during cell culture and purification, Mol. Biotechnol., 36, 151-158 (2007)
Henriques, A. M., Fevereiro, M., Prazeres, D. M. F., Monteiro, G. A., Development of a candidate DNA vaccine against Maedi-Visna virus, Vet. Immunol. Immunopathol., 119, 222-232 (2007)
Lienqueo, M. E., Salazar, O., Henriquez, K., Calado, C. R. C., Fonseca, L. P., Cabral, J. M. S., Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography, J. Chromat. A, 1154, 460463 (2007)
Marques, M. P. C., de Carvalho, C. C. C. R., Claudino, M. J. C., Cabral, J. M. S., Fernandes, P., On the feasibility of the microscale approach for a multi-step biotransformation: sitosterol side-chain cleavage, J. Chem. Technol. Biotechnol., 82, 856-863 (2007)
Madeira, C., Loura, L., Prieto, M., Fedorov, A., Aires-Barros, M. R., Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size, Eur. Biophys. J. Biophys., 36, 609-620 (2007)
Monteiro, G. A., Prazeres, D. M. F., Design and production of DNA vaccines, Bioforum Europe, 12/07, 20-22 (2007)
Mergulhγo, F. J. M., Monteiro, G. A., Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli, J. Microbiol. Biotechnol., 17, 1236-1241 (2007)
Melo, E. P., Fernandes, A. T., Durγo, P., Martins, L. O., Insight into stability of Cot-A laccase from the spore coat of Bacillus subtilis, Biochem. Soc. Trans., 35, 1579-1582 (2007)
Neves-Petersen, M. T., Klitgaard, S., Carvalho, A. S. L., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., Photophysics and photochemistry of horseradish peroxidase A2 upon UV illumination, Biophys. J., 92, 2016-2017 (2007)
Otzen, D. E., Giehm, L., Baptista, R. P., Kristensen, S. R., Melo, E. P. Pedersen, S., Aggregation as the basis for complex behaviour of cutinase in different denaturants, Biochim. Biophys. Acta, 1774, 323-333 (2007)
Pimentel, A. C, Pereira, A. T., Chu, V., Prazeres, D. M. F., Conde, J. P., Detection of chemiluminescence using an amorphous silicon photodiode, IEEE Sensors Lett., 7, 415-416 (2007)
Pistor, P., Prazeres, D. M. F., Chu, V., Conde, J. P., pH sensitive photoresistor based on poly (para- phenylene-vinylene), Sensors Actuators B: Chemical, 123, 153-157 (2007)
Prazeres, D. M .F., Monteiro, G. A., Produηγo de vacinas de DNA, Engenharia Quνmica: Indϊstria, Ciκncia e Tecnologia, 7, 34-39 (2007)
Rosa, P. A. J., Azevedo, A. M., Aires-Barros, M. R., Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies, J. Chromat. A, 1141, 50-60 (2007)
Rosa, P. A. J., Azevedo, A. M., Ferreira, I. F., de Vries, J., Korporaal, R., Verhoef, H. J., Visser, T. J., Aires-Barros, M. R., Affinity partitioning of human antibodies in aqueous two-phase systems, J. Chromat. A, 1162, 103-113 (2007)
Roque, A. C. A., Silva, C. S. O., Taipa, M. A., Affinity-based methodologies and ligands for antibody purification: Advances and Perspectives, J. Chromat. A, 1160, 44-55 (2007)
Sousa, F., Prazeres, D. M. F., Queiroz, J. A., Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography, Biomedical Chromatog., 21, 993-998 (2007)
Sousa, F., Prazeres, D. M. F., Queiroz, J. A., Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography, Arch. Biochem. Biophys., 467, 154-162 (2007)
Teles, F. F., Prazeres, D. M. F., Lima-Filho, J. L., Electrochemical detection of a dengue-related oligonucleotide sequence using ferrocenium as a hybridization indicator, Sensors, 7, 2510-2518 (2007)
Vojinovic, V., Carvalho, R., Lemos, F., Cabral, J. M. S., Fonseca, L. P., Ferreira, B. S., Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds, Biochem. Eng. J., 35, 126-135 (2007)
Viseu, M. I., Melo, E. P., Carvalho, T. I., Correia, R. F., Costa, S. M. B., Unfolding kinetics of ί-Lactoglobulin induced by surfactant and denaturant: A stopped-flow/fluorescence study, Biophys. J., 93, 601-3612 (2007)
Abranches, E., O'Neill, A., Cabral, J. M. S., Schaffer, D. V., Development of quantitative PCR methods to analyse neural progenitor cell culture state, Biotecnol. Appl. Biochem., 44, 1-8 (2006)
Adrega, T., Prazeres, D. M. F., Chu, V., Conde, J. P., Thin-Film silicon MEMS DNA sensors, J. Non-Crystalline Sol., 352, 1999-2003 (2006)
Almeida, C. F., Calado, C. R. C., Bernardino, S. A., Cabral, J. M. S., Fonseca, L. P., A flow injection analysis system for on-line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time, J. Chem. Technol. Biotechnol., 81, 1678-1684 (2006)
Angelova, B., Fernandes, P., Spasova, D., Mutafov, S., Pinheiro, H. M., Cabral, J. M. S., Scanning electron microscopy investigations on bis(2-Ethylhexyl)phthalate treated Mycobacterium cells, Microsc. Res. Tech., 69, 613-617 (2006)
de Assis, S. A., Ferreira, B. S., Fernandes, P., Trevisan, H. C., Cabral, J. M. S., Oliveira, O. M. M. F., Production of low methoxyl pectin using immobilized pectinmethylesterase silica from acerola (Malpighia glabra L.), J. Chem. Technol. Biotechnol., 81, 706-709 (2006)
Boutros, M., Brαs, L. P., Huber, W., Analysis of cell-based RNAi screens, J. Chem. Technol. Biotechnol., 7, R66 (2006)
Brαs, L. P., Menezes, J. C., Dealing with gene expression missing data, IEE Proc. Syst. Biol., 153, 105-119 (2006)
Carvalho, R. H., Lemos, F., Lemos, M. A. N. D. A., Vojinovic, V., Fonseca, L. P., Cabral, J. M. S., Kinetic modelling of phenol co-oxidation using horseradish peroxidase, Bioprocess Biosystems Eng., 29, 99-108 (2006)
Carvalho, R. H., Lemos, F., Lemos, M. A. N. D. A., Cabral, J. M. S., Ribeiro, F. R., Electro-oxidation of phenol on zeolite/graphite composite electrodes: Part 1. Electrochemical behaviour over NaY zeolite, J. Mol. Catal. A: Chemical, 248, 48-52 (2006)
Carvalho, R. H., Lemos, M. A. N. D. A., Lemos, F., Cabral, J. M. S., Ribeiro, F. R., Electro-oxidation of phenol on zeolite/graphite composite electrodes. Part 2. Influence of zeolite type and composition, J. Mol. Catal. A: Chemical, 253, 170-175 (2006)
Chen, L. Y., Ferreira, J. A. B., Costa, S. M. B., Cabrita, G. J. M., Otzen, D. E., Melo, E. P., Compaction of ribosomal protein S6 by sucrose occurs only under native conditions, Biochem., 45, 2189-2199 (2006)
Dνaz, E. G., Catana, R., Ferreira, B. S., Luque, S., Fernandes, P., Cabral, J. M. S., Towards the development of a membrane reactor for enzymatic inulin hydrolysis, J. Memb. Sci., 273, 152-158 (2006)
Fernandes, P., Applied microbiology and biotechnology in the conservation of stone cultural heritage materials, Appl. Microbiol. Biotechnol., 73, 291-296 (2006)
Fernandes, P., Cabral, J. M. S., Microlitre/millilitre shaken bioreactors in fermentative and biotransformation processes - a review, Biocatal. Biotrans., 24, 237-252 (2006)
Ferreira, A. P., Menezes, J. C., Monitoring a complex medium fermentation with sample-sample two-dimensional FT-NIR correlation spectroscopy, Biotechnol. Prog., 22, 866-872 (2006)
Ferreira, B. S., Biotecnologia industrial, Boletim de Biotecnologia, 85, 3-7 (2006)
Freitas, S. S., Santos, J. A. L., Prazeres, D. M. F., Optimization of isopropanol and ammonium sulphate precipitation steps in the purification of plasmid DNA, Biotechnol. Prog., 22, 1179-1186 (2006)
Gonηalves, R., Lobato da Silva, C., Cabral, J. M. S., Zanjani, E. D., Almeida-Porada, G., A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serumfree culture system, Exp. Hematology, 34, 1353-1359 (2006)
Gonηalves, R., Lobato da Silva, C., Ferreira, B. S., Lemos, M. A. N. D. A., Lemos, F., Almeida-Porada, G., Cabral, J. M. S., Kinetic analysis of the ex-vivo expansion of human hematopoietic stem/progenitor cells, Biotechnol. Lett., 28, 335-340 (2006)
Gonηalves, T., Prazeres, D. M. F., Chu, V., Conde, J. P., Label-free electronic detection of biomolecules using a-Si:H Field-effect devices, J. Non-Crystalline Sol., 352, 2007-2010 (2006)
Jaumot, J., Menezes, J. C., Tauler, R., Quality assessment of the results obtained by multivariate curve resolution analysis of multiple runs of gasoline blending processes, J. Chemometrics, 20, 54-67 (2006)
Lopes, V. V., Pinheiro, C. C., Menezes, J. C., Determination of state-space model uncertainty using bootstrap techniques, J. Process Control, 16, 685-692 (2006)
Lobato da Silva, C., Gonηalves, R., Lemos, M. A. N. D. A., Lemos, F., Almeida-Porada, G., Cabral, J. M. S., Ex-vivo expansion and differentiation of human hematopoietic stem/progenitor cells supported by human stromal-based culture systems, Actas de Bioquνmica, 7, 21-31 (2006)
Lourenηo, N. D., Chaves, C. L., Novais, J. M., Menezes, J. C., Pinheiro, H. M., Diniz, D., UV spectra analysis for water quality monitoring in a fuel park wastewater treatment plant, Chemosphere, 65, 786-791 (2006)
Lourenηo, N. D., Chaves, C. L., Menezes, J. C., Pinheiro, H. M., Diniz, D., A espectroscopia ultravioleta-visνvel na prevenηγo de descargas nγo-conformes de αguas residuais industriais, Engenharia Quνmica, 4, 56-62 (2006)
Martins, V., Ferreira, H. A., Fonseca, L. P., Freitas, P. P., Cabral, J. M. S., Biossensores magnetoresistivos e nanomarcadores magnιticos - Monitorizaηγo de microorganismos patogιnicos em αgua, Boletim de Biotecnologia, 83, 11-21 (2006)
Minhalma, M., Beal, L. L., Catarino, I., Mateus, M., de Pinho, M. N., Optimization of saccharide fractionation using nanofiltration/ultrafiltration, Desalination, 199, 337-339 (2006)
Passarinha, L. A., Diogo. M. M., Queiroz, J. A., Monteiro, G. A., Fonseca, L. P., Prazeres, D. M. F., Production of ColE1 type plasmid by Escherichia coli DH5a cultured under non-selective conditions, J. Microb. Biotechnol., 16, 20-24 (2006)
Pedersen, S., Nesgaard, L. Baptista, R. P., Melo, E. P. Kristensen, S. R., Otzen, D. E., pH-dependent aggregation of cutinase is efficiently suppressed by 1,8-ANS, Biopolymers, 83, 619-629 (2006)
Rocha, J. R., Catana, R., Ferreira, B. S., Cabral, J. M. S., Fernandes, P., Design and characterization of an enzyme system for inulin hydrolysis, Food Chem., 95, 77-82 (2006)
Rodrigues, M. L., Archer, M., Martel, P., Miranda, S., Thomaz, M., Enguita, F. J., Baptista, R. P., Melo, E. P., Sousa, N., Cravador, A., Carrondo, M. A., Crystal structures of the free and sterol-bound forms of beta-cinnamomin, Biochim. Biophys. Acta, 1764, 110-121 (2006)
Rodrνguez, V. B., Alameda, E. J., Gallegos, J. F. M., Requena, A. R., Lσpez, A. I. G., Cabral, J. M. S., Fernandes, P., Fonseca, L. J. P., Modification of the activity of an α-amylase from Bacillus licheniformis by several surfactants, Electronic J. Biotechnology, 9, 567-571 (2006)
Ruiu, L., Roque, A. C. A., Taipa, M. A., Lowe, C. R., De novo design, synthesis and screening of a combinatorial library of complementary ligands directed towards the surface of cutinase from Fusarium solani pisi, J. Mol. Recognit., 19, 372-378 (2006)
Silva, C. S. O, Baptista, R.P., Santos, A. M., Martinho, J. M., Cabral, J. M. S., Taipa, M. A., Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles, Biotechnol. Lett., 28, 2019-2025 (2006)
Silva, A. I., Norton-de-Matos, A., Brons, I. G., Mateus, M., An overview on the development of a bio-artificial pancreas as a treatment of insulin-dependent Diabetes Mellitus, Med. Res. Rev., 26, 181-222 (2006)
Sousa, F., Freitas, S. .S. , Azzonni, A., Prazeres, D. M. F., Queiroz, J. A., Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-chromatography step, Biotechnol. App. Biochem., 45, 131-140 (2006)
Valente, C. A., Monteiro, G. A., Cabral, J. M. S., Fevereiro, M., Prazeres, D. M. F., Optimization of the primary recovery of human interferon alpha 2b from Escherichia coli inclusion bodies, Protein Expr. Purif., 45, 226-234, (2006)
Vidinha, P., Augusto, V., Almeida, M., Fonseca, I., Fidalgo, A., Ilharco, L., Cabral, J. M. S., Barreiros, S., Sol-gel encapsulation: an efficient and versatile immobilization technique for cutinase in nonaqueous media, J. Biotechnol., 121, 23-33, (2006)
Vojinovic, V., Bertin, L., Cabral, J. M. S., Fonseca, L. P., Horseradish peroxidase combined with oxidase enzymes a valuable bioanalytical tool: Lactate oxidase a case study, Eng. Life Sci., 6, 181-186, (2006)
Vojinovic, V., Cabral, J. M. S., Fonseca, L. P., Real-time bioprocess monitoring part I: In-situ sensors, Sensors and Actuators B: Chemical, 114, 1083-1091, (2006)
Vojinovic, V., Esteves, F. M. F., Cabral, J. M. S., Fonseca, L. P., Bienzymatic analytical microreactors for glucose, lactate, ethanol, galactose and L-amino acid monitoring in cell culture media, Anal. Chim. Acta, 565, 240-249, (2006)
Angelova, B., Fernandes, P., Cruz, A., Pinheiro, H. M., Mutafov, S., Cabral, J. M. S., Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media, Enzyme Microb. Technol., 37, 718-722 (2005)
Azevedo, A. M., Prazeres, D. M. F., Cabral, J. M. S., Fonseca, L. P., Ethanol biosensors based on alcohol oxidase, Biosens. Bioelectron., 21, 235-247 (2005)
Brαs, L. P., Bernardino, S. A., Lopes, J. A., Menezes, J. C., Multiblock PLS as an approach to compare and combine NIR and MIR spectra in calibrations of soybean flour, Chemom. Intell. Lab. Syst., 75, 91-99 (2005)
Carvalho, A. S. L., Neves-Petersen, M. T., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium, Biochim. Biophys. Acta, 1747, 99-107 (2005)
Carvalho, C. C. R. de, Marques, M. P. C., Fernandes, P., Fonseca, M. M. R. da, A simple imaging method for biomass determination, J. Microb. Meth., 60, 135-140 (2005)
Catana, R., Ferreira, B. S., Cabral, J. M. S., Fernandes, P., Immobilization of inulinase for sucrose hydrolysis, Food Chem., 91, 517-520 (2005)
Chen, L. Y., Cabrita, G. J. M., Otzen, D. E., Melo, E. P., Stabilization of the ribosomal protein S6 by trehalose is counterbalanced by the formation of a putative off-pathway species J. Mol. Biol., 351, 402-416 (2005)
Diogo, M. M., Queiroz, J. A., Prazeres, D. M. F., Chromatography of plasmid DNA, J. Chrom. A, 1069, 3-22 (2005)
Faber, A., Ferreira, G. N. M., Silva, S. N., Rueff, J., Gaspar, J., Multiplex PCR-single-base extension genotyping of multiple glutathione S-transferase polymorphisms, Biotechnol. Appl. Biochem., 41, 9-15 (2005)
Felνcio, C. C., Brαs, L. P., Lopes, J. A., Cabrita, L., Menezes J. C., Comparison of PLS algorithms in gasoline and gas oil parameter monitoring with MIR and NIR, Chemom. Intell. Lab. Syst., 78, 74-80 (2005)
Ferreira, A. P., Alves, T. P., Menezes, J. C., Monitoring complex media fermentations with near-infrared spectroscopy: Comparison of different variable selection methods, Biotechnol. Bioeng., 91, 474-481 (2005)
Ferreira, A. P., Vieira, L. M., Cardoso, J. P., Menezes, J. C., Evaluation of a new annular capacitance probe for biomass monitoring in industrial pilot-scale fermentations, J. Biotechnol., 116, 403-409 (2005)
Fixe, F., Branz, H., Louro, N., Chu, V., Prazeres, D. M. F., Conde, J. P., Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips, Nanotechnology, 16, 2061-2071 (2005)
Fixe, F., Chu, V., Prazeres, D. M. F., Conde, J. P., Single base mismatch detection by microsecond voltage pulses in addressable DNA microarrays, Biosens. Bioelectron., 21, 888-893 (2005)
Garcia, S., Vidinha, P., Arvana, H., Gomes da Silva, M. D. R., Ferreira, M. O., Cabral, J. M. S., Macedo, E. A., Harper, N., Barreiros, S., Cutinase activity in supercritical and organic media: water activity, solvation and acid-base effects, J. Supercritical Fluids, 35, 62-69 (2005)
Lopes, V. V., Menezes, J. C., Inferential sensor design in the presence of missing data: a case study, Chemom. Intell. Lab. Syst., 78, 1-10 (2005)
Madeira, C., Fedorov, A., Aires-Barros, M. R., Prieto, M., Loura, L. M. S., Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes, Photochem. Photobiol., 81, 1450-1459 (2005)
Mergulhγo, F. J. M., Summers, D. K., Monteiro, G. A., Recombinant protein production in Escherichia coli, Biotechnol. Adv., 23, 177-202 (2005)
Moreira, K. A., Diogo, M., Prazeres, D. M. F., Filho, J. L. L., Porto, A. L. F., Purification of plasmid (pVaxLacZ) by Hydrophobic Interaction Chromatography, Brazilian Arch. Biol. Technol., 48, 113-117 (2005)
Rodrigues, L. O., Marques, J. L., Cardoso, J. P., Menezes, J. C., From lab to plant: a scale-independent NIR calibration to monitor an ionic exchange column, Chemom. Intell. Lab. Syst., 75, 101-108 (2005)
Rodrigues, L. O., Marques, J. L., Cardoso, J. P., Menezes, J. C., A PAT application on an API crystallisation process, J. Process Analytical Technology, 2, 12-17 (2005)
Roque, A. C. A., Taipa, M. A., Lowe, C. R., An artificial protein L for the purification of immunoglobulins and Fab-fragments by affinity chromatography, J. Chromat. A, 1064, 157-167, (2005)
Roque, A. C. A., Taipa, M. A., Lowe, C. R., Synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein L from Peptostreptococcus magnus, J. Molecular Recognition, 18, 213-224, (2005)
Silva, C. M., Carneiro, F., O'Neill, A., Fonseca, L. P., Cabral, J. M. S., Guebitz, G., Cavaco-Paulo, A., Cutinase - A new tool for biomodification of synthetic fibers, J. Polym. Sci.: Polym. Chem., 43, 2448-2450, (2005)
da Silva, C. L., Gonηalves, R., Crapnell, K. B., Cabral, J. M. S., Zanjani, E. D., Almeida-Porada, G., A human stromal-based serum free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells, Exp. Hematol., 33, 828835 (2005)
Silva, F. A., Costa, M. J. L., Corte-Real S., Goncalves, J., Cell type-specific targeting with Sindbis pseudotyped lentiviral vectors displaying anti-CCR5 single chains antibodies, Hum. Gene Ther., 16, 223-234, (2005)
Sousa, F., Tomaz, C. T., Prazeres, D. M. F., Queiroz, J. A., Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support, Anal. Biochem., 343, 183-185, (2005)
Teles, F. F., Prazeres, D. M. F., Lima-Filho, J. L., Trends in Dengue Diagnosis, Rev. Med. Virol., 15, 287-302, (2005)
Trindade, I. P., Diogo, M. M., Prazeres, D. M. F., Marcos, J. C., Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography, J. Chrom. A, 1082, 176-184, (2005)
Vojinovic, V., Calado, C. R., Silva, A. I., Mateus, M., Cabral, J. M. S., Fonseca, L. P., Micro-analytical GO/HRP bioreactor for glucose determination and bioprocess monitoring, Biosens. Bioelectron., 10, 1955-1961, (2005)
Wendhausen, R., Frigato, M., Fernandes, P., Carvalho, C. C. C. R., Cruz, A., Pinheiro, H. M., Cabral, J. M. S., Chrysotile as a support for the immobilisation of Mycobacterium sp. NRRL B-3805 cells for the bioconversion of b-sitosterol in an organicaqueous two-liquid phase system, J. Mol. Catal. B: Enzym., 32, 61-65, (2005)
Almeida, C. F., Cabral, J. M. S., Fonseca, L. P., Flow Injection Analysis System for on-line cutinase activity assay, Anal. Chim. Acta, 502, 115-124, (2004)
Azevedo, A. M., Cabral, J. M. S., Gibson, T. D., Fonseca, L. P., Operation and performance of analytical packed-bed reactors with an immobilised alcohol oxidase, J. Mol. Catal. B: Enzym., 28, 45-53, (2004)
Azevedo, A. M., Cabral, J. M. S., Prazeres, D. M. F., Gibson, T. D., Fonseca, L. P., Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase, J. Mol. Catal. B: Enzym., 27, 37-45, (2004)
Azevedo, A. M., Vojinovic, V., Cabral, J. M. S., Gibson, T. D., Fonseca, L. P., Operational stability of immobilised horseradish peroxidase in mini-packed bed bioreactors, J. Mol. Catal. B: Enzym., 28, 121-128, (2004)
Calado, C. R. C., Ferreira, B. S., da Fonseca, M. M. R., Cabral, J. M. S., Fonseca, L. P., Integration of the production and the purification processes of cutinase secreted by a recombinant Saccharomyces cerevisiae SU50 strain, J. Biotechnol., 109, 147-158 (2004)
Cao, X. J., Wu, X. Y., Fonseca, L. J. P., Cabral, J. M. S., Marcos, J. C., Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase, Biotechnol. Lett., 26, 97-101 (2004)
Carvalho, A. S. L., Santos, A. M., Neves-Petersen, M. T., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., Conformational States of HRPA1 Induced by Thermal Unfolding: Effect of Low Molecular Weight Solutes, Biopolymers, 75, 173-186 (2004)
Carvalho, C. C. R. de, Cruz, A., Angelova, B., Fernandes, P., Pons, M. N., Pinheiro, H. M., Cabral, J. M. S., Fonseca, M. M. R. da, Behaviour of Mycobacterium sp. NRRL B-3805 whole cells in aqueous, organic-aqueous and organic media studied by fluorescence microscopy, Appl. Microb. Biotech., 64, 695-701 (2004)
Carvalho, C. C. R. de, Cruz, A. A. R. L., Pons, M. N., Pinheiro, H. M. R. V., Cabral, J. M. S., Fonseca, M. M. R. da, Ferreira, B. S., Fernandes, P., Mycobacterium sp., Rhodococcus erythropolis, and Pseudomonas putida behavior in the presence of organic solvents, Microsc. Res. Tech., 64, 215-222 (2004)
Cruz, A., Angelova, B., Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Study of key operational parameters for the side-chain cleavage of sitosterol by free mycobacterial cells in bis-(2-ethylhexyl) phthalate, Biocatal. Biotrans., 22, 189-194 (2004)
Cruz, A., Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Solvent partitioning and whole-cell sitosterol bioconversion activity in aqueous-organic two-phase systems, Enzyme Microb. Technol., 34, 342-353 (2004)
de Assis, S. A., Ferreira, B. S., Fernandes, P., Trevisan, H. C., Cabral, J. M. S., Oliveira, O. M. M. F., Gellatin immobilized pectinmethylesterase for production of low methoxyl pectin, Food Chem., 86, 333-337 (2004)
de Assis, S. A., Fernandes, P., Ferreira, B. S., Trevisan, H. C., Cabral, J. M. S., Oliveira, O. M. M. F., Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L.), J. Chem. Technol. Biotechnol., 79, 277-280 (2004)
Ferreira, B. S., Abranches, E., Gonηalves, R., Lobato da Silva, C., Cabral, J. M. S., Bioengenharia de Cιlulas Estaminais, Boletim de Biotecnologia, 78, 13-25 (2004)
Ferreira, B. S., Calado, C. R. C., van Keulen, F., Fonseca, L. P., Cabral, J. M. S., da Fonseca, M. M. R., Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways , J. Biotechnol., 109, 159-167 (2004)
Fixe, F., Dufva, M., Telleman, P., Christensen, C. B. V., One-step immobilization of aminated and thiolated DNA onto poly(methylmethacrylate) (PMMA) substrates, Lab-on-Chip, 4, 191-195 (2004)
Fixe, F., Dufva, M., Telleman, P., Christensen, C. B. V., Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays, Nucleic Acids Res., 32, e9 (2004)
Fixe, F., Chu, V., Prazeres, D. M. F., Conde, J. P., An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays, Nucleic Acids Res., 32, e70 (2004)
Fixe, F., Branz, H., Louro, N., Chu, V., Prazeres, D. M. F., Conde, J. P., Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays, Biosens. Bioelectron., 19, 1591-1597 (2004)
Frias, A., Crapnell, K. B., Porada, C., Cabral, J. M. S., Zanjani, E. D., Almeida-Porada, G., Ex-vivo generation and expansion of both lymphoid and myeloid lineages from human cord blood (CB) HSC using a serum-free human mesenchymal stem cell based culture system, Blood, 104, 789A-790A (2004)
Garcia, S., Lourenco, N. M. T., Lousa, D., Sequeira, A. F., Mimoso, P., Cabral, J. M. S., Afonso, C. A. M., Barreiros, S. , A comparative study of biocatalysis in non-conventional solvents: Ionic liquids, supercritical fluids and organic media, Green Chem., 6, 466-470, (2004)
Kerr, S., Calι, C., Cabral, J. M. S., van Keulen, F., Factors enhancing lycopene production by a new Mycobacterium aurum mutant, Biotechnol. Lett., 26, 103-108, (2004)
Lopes, J. A., Costa, P. F., Alves, T. P., Menezes, J. C., Chemometrics in bioprocess engineering: process analytical technology (PAT) applications, Chemom. Intell. Lab. Syst., 74, 269-275 (2004)
Lopes, J. A., Menezes, J. C., Multivariate monitoring of fermentation processes with non-linear modelling methods, Anal. Chim. Acta, 515, 101-108 (2004)
Mergulhγo, F. J. M., Taipa, M. A., Cabral, J. M. S., Monteiro, G. A., Evaluation of bottlenecks in proinsulin secretion by Escherichia coli, J. Biotechnol., 109, 31-43 (2004)
Mergulhγo, F. J. M., Monteiro, G. A., Cabral, J. M. S., Taipa, M. A., Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli, J. Microb. Biotecnol., 14, 1-14 (2004)
Mergulhγo, F. J. M., Monteiro, G. A., Secretion capacity limitations of the Sec pathway in Escherichia coli, J. Microb. Biotecnol., 14, 128-133 (2004)
Prazeres, D. M. F., Ferreira, G. N. M., Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination, Chem. Eng. Proc., 43, 615-630 (2004)
Ribeiro, S. C., Monteiro, G. A., Prazeres, D. M. F., The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases, J. Gene Med., 6, 565-573 (2004)
Roque, A. C. A., Lowe, C. R., Taipa, M. A., Antibodies and genetically engineered related molecules: production and purification, Biotechnol. Prog., 20, 639-654, (2004)
Roque, A. C. A., Taipa, M. A., Lowe, C. R., A new method for the screening of solid-phase combinatorial libraries for affinity chromatography, J. Molecular Recognition, 17, 262-267, (2004)
Sα-Pereira, P., Carvalho, A. S. L., Costa-Ferreira, M., Aires-Barros, M. R., Thermostabilization of Bacillus subtillis CCMI 966 3 xylanases with trehalose. Study of deactivation kinetics, Enzyme Microb. Technol., 34, 278-282, (2004)
Serralha, F. N., Lopes, J. M., Lemos, F., Ramτa Ribeiro, F., Prazeres, D. M. F., Aires-Barros, M. R., Cabral, J. M. S., Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6, J. Mol. Catal. B: Enzym., 27, 19-27, (2004)
Silva, C., Cabral, J. M. S., van Keulen, F., Isolation of a b-carotene overproducing soil bacterium Sphingomonas sp., Biotechnol. Lett., 26, 257-262, (2004)
Staebler, A., Cruz, A., van der Goot, W., Pinheiro, H. M., Cabral, J. M. S., Fernandes, P., Optimization of androstenedione production in an organic-aqueous two-liquid phase system, J. Mol. Catal. B: Enzym., 29, 19-23, (2004)
Valente, C. A., Cabral, J. M. S., Prazeres, D. M. F., Monteiro, G. A., Translational features of human interferon alpha 2b production in Escherichia coli, Appl. Environ. Microbiol., 70, 5033-5036, (2004)
Vidinha, P., Harper, N., Micaelo, N. M., Lourenηo, N. M. T., da Silva, M. D. R. G., Cabral, J. M. S., Afonso, C. A. M., Soares, C. M., Barreiros, S., Effect of immobilization support, water activity, and enzyme ionization state on cutinase activity and enantioselectivity in organic media, Biotechnol. Bioeng., 85, 442-449, (2004)
Vojinovic, V., Azevedo, A. M., Martins, V. C. B., Cabral, J. M. S., Gibson, T. D., Fonseca, L. P., Assay of H2O2 by HRP catalysed co-oxidation of phenol-4-sulphonic acid and 4-aminoantipyrine: Characterisation and optimisation, J. Mol. Catal. B: Enzym., 28, 129-135, (2004)
Abranches, E., Bekman, E., Henrique, D., Cabral, J. M. S., Expansion and neural differentiation of embryonic stem cells in adherent and suspension cultures, Biotechnol. Lett., 25, 725-730, (2003)
Alves, J. R. F., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Optimisation of penicillin acylase extraction by AOT/isooctane reversed micellar systems, Biochem. Eng. J., 15, 81-86, (2003)
Baptista, R. P., Chen, L. Y., Paixγo, A., Cabral, J. M. S., Melo, E. P., A novel pathway to enzyme deactivation: The cutinase model, Biotechnol. Bioeng., 82, 851-857 (2003)
Baptista, R. P., Santos, A. M., Fedorov, A., Martinho, J. M. G., Pichot, C., Elaοssari, A., Cabral, J. M. S., Taipa, M. A., Activity, conformation and dynamics of cutinase adsorbed on polymethyl(metha)acrylate particles, J. Biotechnol., 102, 241-249 (2003)
Cabrita, G. J. M., Ferreira, B. S., Silva, C. A. M. L., Gonηalves, R. M., Almeida-Porada, G., Cabral, J. M. S., Hematopoietic stem cells: from the bone to the bioreactor, Trends Biotechnol., 21, 233-240 (2003)
Calado, C. R. C., Almeida, C., Cabral, J. M. S., Fonseca, L. P., Development of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strain, J. Biosci. Bioeng., 96, 141-148 (2003)
Carvalho, A. S. L., Melo, E. P., Ferreira, B. S., Neves-Petersen, M. T., Petersen, S. B., Aires-Barros, M. R., Heme and pH-dependent stability of an anionic horseradish peroxidase , Arch. Biochem. Biophys., 415, 257-267 (2003)
Carvalho, C. C. R. de, Fonseca, M. M. R. da, A Simple Method to Observe Organic Solvent Drops with a Standard Optical Microscope, Microsc. Res. Tech., 60, 465-466 (2003)
Carvalho, C. C. R. de, Fonseca, M. M. R. da, Towards the bio-production of trans-carveol and carvone from limonene: induction after cell growth on limonene and toluene, Tetrahedron: Asymmetry, 14, 3925-3931 (2003)
Carvalho, C. C. R. de, Pons, M.-N., Fonseca, M. M. R. da, Principal Components Analysis as a Tool to Summarise Biotransformation Data: Influence on Cells of Solvent Type and Phase Ratio, Biocatal. Biotrans., 21, 305-314 (2003)
Cunha, M. T., Costa, M. J. L., Calado, C. R. C., Fonseca, L. P., Aires-Barros, M. R., Cabral, J. M. S., Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins, J. Biotechnol, 100, 55-64 (2003)
Diogo, M. M., Queiroz, J. A., Prazeres, D. M. F., Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography, J. Chrom. A, 998, 109-117 (2003)
Diogo, M. M., Prazeres, D. M. F., Pinto, N. G., Queiroz, J. A., Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B using and relating two different models for describing the effect of salt and temperature on retention, J. Chrom. A, 1006, 137-148 (2003)
Fernandes, P., Cruz, A., Angelova, B., Pinheiro, H. M., Cabral, J. M. S., Microbial conversion of steroid compounds: recent developments, Enzyme Microb. Technol., 32, 688-705 (2003)
Fernandes, P., Ferreira, B. S., Cabral, J. M. S., Solvent tolerance in bacteria: role of efflux pumps and cross-resistance with antibiotics, Int. J. Antimicrob. Agents, 2, 211-216 (2003)
Ferreira, B. S., Calado, C. R. C., van Keulen, F., Fonseca, L. P., Cabral, J. M. S., da Fonseca, M. M. R., Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects, Appl. Microb. Biotech., 61, 69-76 (2003)
Ferreira, H. A., Graham, D. L., Freitas, P. P., Cabral, J. M. S., Biodetection using magnetically labeled biomolecules and arrays of spin valve sensors, J. Appl. Phys., 93, 7281-7286 (2003)
Ferreira-Dias, S., Correia, A. C., Fonseca, M. M. R. da, Response surface modelling of glycerolysis catalysed by Candida rugosa lipase immobilised in different polyurethane foams for the production of partial glycerides, J. Mol. Catal. B: Enzym., 21, 71-81 (2003)
Fixe, A., Cabeηa, R., Prazeres, D. M. F., Ferreira, G. N. M., Chu, V, Conde, J. P., Electric field pulse assisted covalent immobilization of DNA in the nanosecond time scale, Appl. Phys. Lett., 83, 1465-1467 (2003)
Freitas, P. P., Ferreira, H., Graham, D., Clarke, L., Amaral, M., Martins, V., Fonseca, L., Cabral, J. S., Magnetoresistive biochips, Europhysics News, 34, 224-226 (2003)
Gonηalves, A. M. D., Aires-Barros, M. R., Cabral, J. M. S., Interaction of an anionic surfactant with a recombinant cutinase from Fusarium solani pisi: a spectroscopic study, Enzyme Microb. Technol., 32, 868-879 (2003)
Graham, D. L., Ferreira, H. A., Freitas, P. P., Cabral, J. M. S., High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors, Biosen. Bioelectron., 18, 483-488 (2003)
Leiriγo, P. R. S., Fonseca, L. J. P., Taipa, M. A., Cabral, J. M. S., Mateus, M., Horseradish peroxidase immobilized through its carboxylic groups onto a polyacrylonitrile membrane. Comparison of enzyme performances with inorganic beaded supports, Appl. Biochem. Biotechnol., 110, 1-10 (2003)
Lobato da Silva, C., Gonηalves, R., Lemos, F., Lemos, M. A. N. D. A., Zanjani, E. D., Almeida-Porada, G., Cabral, J. M. S., Modelling of ex vivo expansion/maintenance of hematopoietic stem cells, Bioprocess Biosystems Eng., 25, 365-369 (2003)
Lopes, J. A., Menezes, J. C., Industrial Fermentation End-Product Modelling with Multilinear PLS, Chemom. Intell. Lab. Syst., 68, 75-81 (2003)
Madeira, C., Loura, L., Aires-Barros, R., Fedorov, A., Prieto, M., Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer , Biophys. J., 85, 3106-3119 (2003)
Martinho, J. M. G., Santos, A. M., Fedorov, A., Baptista, R. P., Taipa, M. A., Cabral, J. M. S., Fluorescence of the single tryptophan of cutinase: Temperature and pH effect on protein conformation and dynamics, Photochem. Photobiol., 78, 15-22 (2003)
Martins, S. A. M., Prazeres, D. M. F., Cabral, J. M. S., Monteiro, G. A., Comparison of real-time PCR and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products, Anal. Biochem., 322, 127-129 (2003)
Melo, E. P., Baptista, R. P., Cabral, J. M. S., Improving cutinase stability in aqueous solution and in reverse micelles by media engineering, J. Mol. Catal. B: Enzym., 22, 299-306 (2003)
Melo, E. P., Chen, L. Y., Cabral, J. M. S., Fojan, P., Petersen, S. B., Otzen, D. E., Trehalose favors a cutinase compact intermediate off-folding pathway, Biochem., 42, 7611-7617 (2003)
Melo, E. P., Costa, S. M. B., Cabral, J. M. S., Fojan, P., Petersen, S. B., Cutinase-AOT interactions in reverse micelles: the effect of 1-hexanol, Chem. Phys. Lipids, 124, 37-47 (2003)
Mergulhγo, F. J. M., Monteiro, G. A., Larsson, G., Bostrom, M., Farewell, A., Nystrom, T., Cabral, J. M. S., Taipa, M. A., Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli, Biotechnol. Appl. Biochem., 38, 87-93 (2003)
Mergulhγo, F. J. M., Monteiro, G. A., Larsson, G., Sandιn, A. M., Farewell, A., Nystrom, T., Cabral, J. M. S, Taipa, M. A., Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB, Appl. Microbiol. Biotechnol., 61, 495-501 (2003)
Sα-Pereira, P., Paveia, H., Costa-Ferreira, M., Aires-Barros, M. R., A new look at xylanases: An overview of purification strategies, Mol. Biotechnol., 24, 257-282 (2003)
Sousa, A. C. C., Mateus, M., Application of factorial design to microfiltration performance. Study of electrostatic and hydrodynamic effects, Chem. Eng. Res. Des., 81, 271-276 (2003)
Vasconcelos, J. M. T., Rodrigues, J. M. L., Orvalho, S. C. P., Alves, S. S., Mendes, R. L., Reis, A., Effect of contaminants on mass transfer coefficients in bubble column and airlift contactors, Chem. Eng. Sci., 58, 1431-1440 (2003)
Alves, S. S., Maia, C. I., Vasconcelos, J. M. T., Experimental and modelling study of gas dispersion in a double turbine stirred tank, Chem. Eng. Sci., 57, 487-496 (2002)
Alves, S. S., Maia, C. I., Vasconcelos, J. M. T., Serralheiro, A. J., Bubble size in aerated stirred tanks, Chem. Eng. J., 89, 109-117 (2002)
Amaral, J., Ingaρas, M., Cabral, J. M. S., Prazeres, D. M. F., Study on the scale-up of human IgG3 purification using protein A affinity chromatography, Bioseparation, 10, 139-143 (2002)
Calado, C. R. C., Cabral, J. M. S., Fonseca, L. P., Effect of Saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase, J. Chem. Technol. Biotechnol., 77, 1231-1237 (2002)
Calado, C. R. C., Mannesse, M., Egmond, M., Cabral, J. M. S., Fonseca, L. P., Production of wild type and peptide fusion cutinases by recombinant Saccharomyces cerevisiae MM01 strains, Biotechnol. Bioeng., 78, 692-698 (2002)
Calado, C. R. C., Monteiro, S. M. S., Cabral, J. M. S., Fonseca, L. P., Effect of pre-fermentation on the production of cutinase by a recombinant Saccharomyces cerevisiae, J. Biosci. Bioeng., 93, 354-359 (2002)
Calado, C. R. C., Taipa, M. A., Cabral, J. M. S., Fonseca, L. P, Optimization of culture conditions and characterization of cutinase produced by recombinant Saccharomyces cerevisiae, Enzyme Microb. Technol., 31, 161-170 (2002)
Carneiro-da-Cunha, M. G., Rocha, J. M. S., Cabral, J. M. S., Gil, M. H., Garcia, F. A. P., Covalent immobilisation of lipase on different supports, Lat. Am. Appl. Res., 32, 69-72 (2002)
Carvalho, C. C. R. de, Fonseca, M. M. R. da, Influence of Reactor Configuration on the Production of Carvone from Carveol by Whole Cells of Rhodococcus erythropolis DCL14, J. Mol. Catal. B: Enzym., 19-20, 377-387 (2002)
Carvalho, C. C. R. de, Fonseca, M. M. R. da, Maintenance of cell viability in the biotransformation of (-)-carveol with whole cells of Rhodococcus erythropolis, J. Mol. Catal. B: Enzym., 19-20, 389-398 (2002)
Carvalho, C. C. R. de, van Keulen, F., Fonseca, M. M. R. da, Modelling the bio-kinetic resolution of diastereomers present in unequal initial amounts, Tetrahedron: Asymmetry, 13, 1637-1643 (2002)
Chaves, A. C., Silveira, E., Bezerra, R. P., Moreira, K. A., Lucena-Silva, N. L. C., Abath, F. G. C., Porto, A. L. F., Cabral, J. M. S., Lima, J. L., Influence of partition parameters on a recombinant antigen of Schistosoma mansoni expressed on E. coli using poly(ethylene glycol)-hydroxypropyl starch aqueous two-phase system, World J. Microb. Biotech., 18, 645-648 (2002)
Cruz, A., Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Effect of phase composition on the whole-cell bioconversion of b-sitosterol in biphasic media, J. Mol. Catal. B: Enzym., 19, 371-375 (2002)
Cunha, M. T., Aires-Barros, M. R., Large scale extraction of proteins, Mol. Biotechnol., 20, 29-40 (2002)
Dias, A. C. P., Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Isolation of a biodegradable sterol-rich fraction from industrial wastes, Bioresource Technol., 82, 253-260 (2002)
Diogo, M. M., Queiroz, J. A., Prazeres, D. M. F., Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized sepharose CL-6B. Application of the solvophobic theory, J. Chromat. A, 944, 119-128 (2002)
Diogo, M. M., Queiroz, J. A., Prazeres, D. M. F., Studies on the retention of plasmid DNA and Escherichia coli nucleic acids by hydrophobic interaction chromatography, Bioseparation, 10, 211-220 (2002)
Fernandes, P., Vidinha, P., Ferreira, T., Silvestre, H., Cabral, J. M. S., Prazeres, D. M. F., Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media, J. Mol. Catal. B: Enzym., 19, 353-361 (2002)
Ferreira, B. S., van Keulen, F., Fonseca, M. M. R. da, A microporous membrane interface for the monitoring of dissolved and volatile compounds by mass spectrometry, J. Memb. Sci., 208, 49-56 (2002)
Fonseca, L. P., Cabral, J. M. S., An integrated downstream processing strategy for the recovery and partial purification of penicillin acylase from crude media, J. Chem. Technol. Biotechnol., 77, 1176-1185 (2002)
Fonseca, L. P., Cabral, J. M. S., Penicillin acylase release from Escherichia coli cells by mechanical cell disruption and permeabilization, J. Chem. Technol. Biotechnol., 77, 159-167 (2002)
Gonηalves, I., Henriques, A., Raimundo, A., Picanηo, I., Reis, A., Correia Jr., E., Santos, E., Nogueira, P., Osσrio-Almeida, L., Fetal hemoglobin elevation in Hb lepore heterozygotes and its correlation with b-globin cluster linked determinants, Am. J. Hematol., 69, 95-102 (2002)
Graham, D. L., Ferreira, H. A., Bernardo, J., Freitas, P. P., Cabral, J. M. S., Single magnetic microsphere placement and detection on-chip using current line designs with integrated spin valve sensors: Biotechnological applications, J. Appl. Phys., 91, 7786-7788 (2002)
Lιon, R., Prazeres, D. M. F., Molinari, F., Cabral, J. M. S., Microbial stereoselective oxidation of 2-methyl-1,3-propanediol to (R)-b-hydroxyisobutyric acid in aqueous/organic biphasic systems, Biocatal. Biotrans., 20, 201-207 (2002)
Lopes, J. A., Menezes, J. C., Westerhuis, J. A., Smilde, A. K., Multiblock PLS analysis of an industrial pharmaceutical process, Biotechnol. Bioeng., 80, 419-427 (2002)
Marcos, J. C., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Application of surface response analysis to the optimization of penicillin acylase purification in aqueous two-phase systems, Enzyme Microb. Technol., 31, 1006-1014 (2002)
Pedro, A. J. E., Fevereiro, P. S., Serralheiro, M. L., Aires-Barros, M. R., Study of the stability of Vaccinium myrtillus peroxidases in reversed micellar systems, Biocatal. Biotrans., 20, 129-135 (2002)
Qin, Y., Cabral, J. M. S., Properties and new applications of urease, Biocatal. Biotrans., 20, 1-14 (2002)
Ribeiro, S. C., Monteiro, G. A., Cabral, J. M. S., Prazeres, D. M. F., Isolation of plasmid DNA from cell lysates by aqueous two-phase systems, Biotechnol. Bioeng., 78, 376-384 (2002)
Rodrigues, A., Cabral, J. M. S., Taipa, M. A., Immobilization of Chromobacterium viscosum lipase on Eugragit S-100: coupling, characterization and kinetic application in organic and biphasic media, Enzyme Microb. Technol., 31, 133-141 (2002)
Sα-Pereira, P., Costa-Ferreira, M., Aires-Barros, M. R., Enzymatic properties of a neutral endo-1,3(4)-b-xylanase Xyl II from Bacillus subtilis, J. Biotechnol., 94, 265-275 (2002)
Sα-Pereira, P., Mesquita, A., Duarte, J. C., Aires-Barros, M. R., Costa-Ferreira, M., Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties, Enzyme Microb. Technol., 30, 924-933 (2002)
Serralha, F. N., Lopes, J. M., Aires-Barros, M. R., Prazeres, D. M. F., Cabral, J. M. S., Lemos, F., Ramτa Ribeiro, F., Stability of a recombinant cutinase immobilized on zeolites, Enzyme Microb. Technol., 31, 29-34 (2002)
Sousa, A. C., Cabral, J. M. S., Mateus, M., Microfiltration of cutinase and Escherichia coli cell fragment suspensions. The role of the electrolyte environment on the development of dynamic cake layers, J. Memb. Sci., 207, 171-187 (2002)
Tambourgi, D. V., Silva, M. S., Billington, S. J., de Andrade, R. M., Magnoli, F. C., Songer, J. G., Van Den Berg, C. W., Mechanism of induction of complement susceptibility of erythrocytes by spider and bacterial sphingomyelinases, Immunology, 107, 93-101 (2002)
Vasconcelos, J. M. T., Orvalho, S. P., Alves, S. S., Gas-liquid mass transfer to single bubbles: effect of surface contamination, AIChE J., 48, 1145-1154 (2002)
Azevedo, A. M., Fonseca, L. P., Graham, D. L., Cabral, J. M. S., Prazeres, D. M. F., Behaviour of horseradish peroxidase in AOT reversed micelles, Biocatal. Biotrans., 19, 213-233 (2001)
Azevedo, A. M., Prazeres, D. M. F., Cabral, J. M. S., Fonseca, L. P., Stability of free and immobilised peroxidase in aqueous mixtures of organic solvents, J. Mol. Catal. B: Enzym., 15, 147-153 (2001)
Borreguero, I., Carvalho, C. M. L., Cabral, J. M. S., Sinisterra, J. V., Alcαntara, A. R., Enantioselective properties of Fusarium solani pisi cutinase on transesterification of acyclic diols: Activity and stability evaluation, J. Mol. Catal. B: Enzym., 11, 613-622 (2001)
Cabral, J. M. S., Ex vivo expansion of hematopoietic stem cells in bioreactors, Biotechnol. Lett., 23, 741-751 (2001)
Calado, C. R. C., Hamilton, G. E., Fonseca, L. P., Cabral, J. M. S., Lyddiatt, A., Direct product sequestration of a recombinant cutinase from batch fermentations of Saccharomyces cerevisiae, Bioseparation, 10, 87-97 (2001)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., A continuous membrane bioreactor for ester synthesis in organic media: I. Operational characterization and stability, Biotechnol. Bioeng., 72, 127-135 (2001)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., A continuous membrane bioreactor for ester synthesis in organic media: II. Modeling of MBR continuous operation, Biotechnol. Bioeng., 72, 136-143 (2001)
Cruz, A., Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Whole-cell bioconversion of b-sitosterol in aqueous-organic two-phase systems, J. Mol. Catal. B: Enzym., 11, 579-585 (2001)
Diogo, M. M., Ribeiro, S. C., Queiroz, J. A., Monteiro, G. A., Perrin, P., Tordo, N., Prazeres, D. M. F., Production, purification and analysis of an experimental DNA vaccine against rabies, J. Gene Medicine, 3, 577-584 (2001)
Domingues, M. F., Queiroz, J. A., Cabral, J. M. S., Fonseca, L. P., Production of cellulases in batch culture using a mutant strain of Trichoderma reesei growing on soluble carbon source, Biotechnol. Lett., 23, 771-775 (2001)
Ferreira-Dias, S., Correia, A. C., Baptista, F. O., Fonseca, M. M. R. da, Contribution of response surface design to the development of glycerolysis systems catalysed by commercial immobilised lipases, J. Mol. Catal. B: Enzym., 11, 699-711 (2001)
Lιon, R., Prazeres, D. M. F., Fernandes, P., Molinari, F., Cabral, J. M. S., A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (R)-b-hydroxyisobutyric acid, Biotechnol. Prog., 17, 468-473 (2001)
Llanes, N., Fernandes, P., Leσn, R., Cabral, J. M. S., Pinheiro, H. M., Conversion of b-sitosterol by Mycobacterium sp. NRRL B-3805 cells immobilized on celite supports, J. Mol. Catal. B: Enzym., 11, 523-530 (2001)
Lobato, C., Gonηalves, R., Lemos, M. A. N. D. A., Lemos, F., Brown, R., McNiece, I. K., Cabral, J. M. S., Zanjani, E. D., Almeida-Porada, M. G., Stromal based serum culture system supports ex-vivo expansion/maintenance of bone marrow (BM) and cord blood (CB) hematopoietic stem cells, Blood, 98, 654a (2001)
Melo, E. P., Faria, T. Q., Martins, L. O., Gonηalves, A. M., Cabral, J. M. S., Cutinase unfolding and stabilization by trehalose and mannosylglycerate, Proteins: Structure, Function and Genetics, 42, 542-552 (2001)
Mergulhγo, F. J. M., Monteiro, G. A., Cabral, J. M. S., Taipa, M. A., A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system, Mol. Biotechnol., 19, 239-244 (2001)
Neves, A. A., Vieira, L. M., Menezes, J. C., Effects of preculture variability on clavulanic acid fermentation, Biotechnol. Bioeng., 72, 628-633 (2001)
Oliveira, A. C., Rosa, M. F., Aires-Barros, M. R., Cabral, J. M. S., Enzymatic esterification of ethanol and oleic acid - a kinetic study, J. Mol. Catal. B: Enzym., 11, 999-1005 (2001)
Oliveira, A. C., Rosa, M. F., Cabral, J. M. S., Aires-Barros, M. R., Effect of extraction and enzymatic esterification on glucose consumption by two Saccharomyces cerevisiae strains: comparative study, J. Chem. Technol. Biotechnol., 76, 285-290 (2001)
Osσrio, N. M., Ferreira-Dias, S., Gusmγo, J. H., Fonseca, M. M. R. da, Response surface modelling of the production of w-3 polyunsaturated fatty acids enriched fats by a commercial immobilized lipase, J. Mol. Catal. B: Enzym., 11, 677-686 (2001)
Queiroz, J. A., Tomaz, C. T., Cabral, J. M. S., Hydrophobic interaction chromatography of proteins, J. Biotechnol., 87, 143-159 (2001)
Serralha, F. N., Lopes, J. M., Lemos, F., Prazeres, D. M. F., Aires-Barros, M. R., Cabral, J. M. S., Ramτa Ribeiro, F., Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite, J. Mol. Catal. B: Enzym., 11, 713-718 (2001)
Serralha, F. N., Lopes, J. M., Vieira Ferreira, L. F., Lemos, F., Prazeres, D. M. F., Aires-Barros, M. R., Cabral, J. M. S., Ramτa Ribeiro, F., Conformational changes induced by immobilization of a recombinant cutinase on zeolites, Catalysis Lett., 73, 63-66 (2001)
Stazi, S. R., Fevereiro, P. S., Sermanni, G. G., Aires-Barros, M. R., Oxidative polymerization and partial dechlorination of 2,4,6-trichlorophenol by a mixture of peroxidase isozymes from Vaccinium mirtyllus, J. Chem. Technol. Biotechnol., 76, 210-214 (2001)
Tecelγo, C. S. R., van Keulen, F., Fonseca, M. M. R. da, Development of a reaction system for the selective conversion of (-)-trans-carveol to (-)-carvone with whole cells of Rhodococcus erythropolis DCL14, J. Mol. Catal. B: Enzym., 11, 719-724 (2001)
Teles, F. R. R., Cabral, J. M. S, Santos, J. A. L, Enzymatic degreasing of a solid waste from the leather industry by lipases, Biotechnol. Lett., 23, 1159-1163 (2001)
Azevedo, A. M., Fonseca, L. P., Prazeres, D. M. F., Kinetic and stability studies of penicillin acylase in reversed micelles, Biocatal. Biotrans., 17, 401-415 (2000)
Baptista, R. P., Cabral, J. M. S., Melo, E. P., Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase, Biotechnol. Bioeng., 70, 699-703 (2000)
Cabral, P. M. P., Lima-Costa, M. E., Cabral, J. M. S., A structured growth model for Cynara cardunculus cell suspension, Bioprocess Eng., 23, 199-203 (2000)
Carvalho, C. C. R. de, van Keulen, F., Fonseca, M. M. R. da, Production and recovery of limonene-1,2-diol and simultaneous resolution of a diastereomeric mixture of limonene-1,2-epoxide with whole cells of Rhodococcus erythropolis DCL14, Biocatal. Biotrans., 18, 223-235 (2000)
Carvalho, C. C. R. de, van Keulen, F., Fonseca, M. M. R. da, Biotransformation of limonene-1,2-epoxide to limonene-1,2-diol by Rhodococcus erythropolis cells. An introductory approach to selective hydrolysis and separation of products, Food Technol. Biotechnol., 38, 181-185 (2000)
Carvalho, C. M. L., Cunnah, P., Aires-Barros, M. R., Cabral, J. M. S., Performance of a membrane bioreactor for enzymatic transesterification: characterization and comparison with a batch stirred tank reactor, Biocatal. Biotrans., 18, 31-57 (2000)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., An integrated model for enzymatic reactions in reversed micellar systems: nominal and effective concentrations, Langmuir, 16, 3082-3092 (2000)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: Modelling of a batch stirred tank reaction, J. Biotechnol., 81, 1-13 (2000)
Carvalho, C. M. L., Cabral, J. M. S., Reverse micelles as reaction media for lipases, Biochimie, 82, 1063-1085 (2000)
Chaves, A. C., Lucena-Silva, N., Abath, F. G. C., Pereira, V. R. A., Lima-Filho, J. L., Porto, A. L. F., Cabral, J. M. S., Liquid-liquid extraction of a Schistosoma mansoni recombinant protein from an Escherichia coli extract in aqueous two-phase system, Bioprocess Eng., 23, 435-438 (2000)
Costa, M. J. L., Cunha, M. T., Cabral, J. M. S., Aires-Barros, M. R., Scale-up of recombinant cutinase whole broth extraction with PEG-phosphate aqueous two phase system, Bioseparation, 9, 231-238 (2000)
Cunha, M. T., Cabral, J. M. S., Tjerneld, F., Aires-Barros, M. R., Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch, Bioseparation, 9, 203-209 (2000)
Diogo, M. M., Queiroz, J. A., Monteiro, G. A., Martins, S. A. M., Ferreira, G. N. M., Prazeres, D. M. F., Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography, Biotechnol. Bioeng., 68, 576-583 (2000)
Diogo, M. M., Ribeiro, S., Queiroz, J. A., Monteiro, G. A., Perrin, P., Tordo, N., Prazeres, D. M. F., Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies, Biotechnol. Lett., 22, 1397-1400 (2000)
Domingues, F. C., Queiroz, J. A., Cabral, J. M. S., Fonseca, L. P., The influence of culture conditions on mycelial structure and cellulase production by Thrichoderma reesei Rut C-30, Enzyme Microb. Technol., 26, 394-401 (2000)
Feliciano, A. S., Dias, A. I. J., Prazeres, D. M. F., The effect of stirring and seeding on the AcPheLeuNH2 synthesis and crystallization in a reversed micellar system, Enzyme Microb. Technol., 27, 264-269 (2000)
Ferreira, B. S., van Keulen, F., Fonseca, M. M. R. da, On-line monitoring of dissolved gases using microporous membrane inlet and mass spectrometry, Food Technol. Biotechnol., 38, 63-67 (2000)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Anion-exchange purification of plasmid DNA using expanded bed adsorption, Bioseparation, 9, 1-6 (2000)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Studies on the batch adsorption of plasmid DNA on to anion-Exchange chromatographic supports, Biotechnol. Prog., 16, 416-424 (2000)
Ferreira, G. N. M., Monteiro, G. A., Prazeres, D. M. F., Cabral, J. M. S., Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications, Trends Biotechnol., 18, 380-388 (2000)
Giordano, R. L. C., Giordano, R. C., Prazeres, D. M. F., Cooney, C. L., Analysis of a Taylor-Poiseuille vortex flow reactor - II: Reactor modelling and performance assessment using glucose-fructose isomerization as test reaction, Chem. Eng. Sci., 55, 3611-3626 (2000)
Gonηalves, A. M., Serro, A. P., Aires-Barros, M. R., Cabral, J. M. S., Effects of ionic surfactants used in reversed micelles on cutinase activity and stability, Biochim. Biophys. Acta, 1480, 92-106 (2000)
Lιon, R., Molinari, F., Prazeres, D. M. F., Cabral, J. M. S., Organic media selection for the extraction of b-hydroxyisobutiric acid produced by microbial biotransformation, J. Chem. Technol. Biotechnol., 75, 617-624 (2000)
Melo, E. P., Fojan, P., Cabral, J. M. S., Petersen, S. B., Dynamic light scattering of cutinase in AOT reversed micelles, Chem. Phys. Lipids, 106, 181-189 (2000)
Melo, E. P., Taipa, M. A., Castellar, M. R., Costa, S. M. B., Cabral, J. M. S., A spectroscopy analysis of thermal stability of the Chromobacterium viscosum, Biophys. Chem., 87, 111-120 (2000)
Mergulhγo, F. J. M., Monteiro, G. A., Kelly, A. G., Taipa, M. A., Cabral, J. M. S., Recombinant human proinsulin: A new approach in gene assembly and protein expression, J. Microbiol. Biotechnol., 10, 690-693 (2000)
Neves, A. A., Pereira, D. A., Vieira, L. M., Menezes, J. C., Real-time monitoring biomass concentration in Streptomyces clavuligerus cultivations with industrial media using a capacitance probe, J. Biotechnol., 84, 45-52 (2000)
Oliveira, A. C., Rosa, M. F., Aires-Barros, M. R.,Cabral, J. M. S., Enzymatic esterification of ethanol by an immobilised Rhizomuchor miehei lipase in a perforated disc bioreactor, Enzyme Microb. Technol., 26, 446-450 (2000)
Porto, A. L. F., Sarubbo, L. A., Lima-Filho, J. L., Aires-Barros, M. R., Cabral, J. M. S., Tambourgi, E. B., Hydrodynamics and mass transfer in aqueous two-phase protein extraction using a continuous perforated rotating disc contactor, Bioprocess Eng., 22, 215-218 (2000)
Ribeiro, S., Monteiro, G. A., Martinho, G., Cabral, J. M. S., Prazeres, D. M. F., Quantitation of plasmid DNA in aqueous two phase systems by fluorescence analysis, Biotechnol. Lett., 22, 1101-1104 (2000)
Serralha, F. N., Lopes, J. M., Lemos, F., Prazeres, D. M. F., Aires-Barros, M. R., Rocha, J., Cabral, J. M. S., Ramτa-Ribeiro, F., Titanosilicates as supports for an enzymatic alcoholysis reaction, React. Kinet. Catal. Lett., 69, 217-222 (2000)
Taipa, M. A., Kaul, R. H., Mattiasson, B., Cabral, J. M. S., Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100, Bioseparation, 9, 291-298 (2000)
Vasconcelos, J. M. T., Orvalho, S. C. P., Rodrigues, A. M. A. F., Alves, S., The effect of blade shape on the performance of six-bladed disk turbine impellers, Ind. Eng. Chem. Res., 39, 203-213 (2000)
Azevedo, A. M., Fonseca, L. P., Prazeres, D. M. F., Stability and stabilisation of penicillin acylase, J. Chem. Technol. Biotechnol., 74, 1110-1116 (1999)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., Cutinase: From molecular level to bioprocess development, Biotechnol. Bioeng., 66, 17-34 (1999)
Carvalho, C. M. L., Cabral, J. M. S., Aires-Barros, M. R., Cutinase stability in AOT reversed micelles: system optimization using the factorial design methodology, Enzyme Microb. Technol., 24, 569-576 (1999)
Carvalho, C. M. L., Melo, E. P., Costa, S. M. B., Cabral, J. M. S., Aires-Barros, M. R., A steady-state fluorescence study of cutinase microencapsulated in AOT reversed micelles at optimal stability conditions, Biotechnol. Lett., 21, 673-681 (1999)
Chaves, A. C., Abath, F. G. C., Lima-Filho, J. L., Cabral, J. M. S., Lucena-Silva, N., Studies on growth kinetics and plasmid stability of a recombinant Escherichia coli expressing a Schistosoma mansoni antigen, Bioprocess Eng., 21, 355-361 (1999)
Diogo, M. M., Queiroz, J. A., Monteiro, G. A., Prazeres, D. M. F., Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography, Anal. Biochem., 275, 122-124 (1999)
Diogo, M. M., Silva, S., Cabral, J. M. S., Queiroz, J. A., Hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilised on Sepharose, J. Chromat. A , 849, 413-419 (1999)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications, Biotechnol. Prog., 15, 725-731 (1999)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Monitoring of plasmid DNA production and purification for gene therapy applications, Pharmacy Pharmac. Commun., 5, 57-59 (1999)
Fonseca, L. P., Cabral, J. M. S., Optimization of a pseudo-affinity process for penicillin acylase purification, Bioprocess Eng., 20, 513-524 (1999)
Gonηalves, A. M., Schacht, E., Matthijs, G., Aires-Barros, M. R., Cabral, J. M. S., Gil, M. H., Stability studies of a recombinant cutinase immobilized to dextran and derivatized silica supports, Enzyme Microb. Technol., 24, 60-66 (1999)
Krieger, N., Taipa, M. A., Melo, E. H. M., Lima-Filho, J. L., Aires-Barros, M. R., Cabral, J. M. S., Purification of a Penicillium citrinum lipase by chromatographic processes, Bioprocess Eng., 20, 59-65 (1999)
Marcos, J. C., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Partial purification of penicillin acylase from Escherichia coli in poly(ethylene glycol)-sodium citrate aqueous two-phase systems, J. Chromat. B, 734, 15-22 (1999)
Mateus, D. M. R., Alves, S. S., Fonseca, M. M. R. da, Diffusion in cell-free and cell immobilising k-carrageenan gel beads with and without chemical reaction, Biotechnol. Bioeng., 63, 625-631 (1999)
Mergulhγo, F. J. M., Kelly, A. G., Monteiro, G. A., Taipa, M. A., Cabral, J. M. S., Troubleshooting in gene splicing by overlap extension: A stepwise method, Mol. Biotechnol., 12, 285-287 (1999)
Molinari, F., Gandolfi, R., Aragozzini, F., Lιon, R., Prazeres, D. M. F., Biotransformations in two-liquid phase systems: production of phenylacetaldehyde by oxidation of 2-phenylethanol with acetic acid bacteria, Enzyme Microb. Technol., 25, 729-735 (1999)
Molinari, F., Villa, R., Aragozzini, F., Lιon, R., Prazeres, D. M. F., Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation, Tetrahedron: Asymmetry, 10, 3003-3009 (1999)
Monteiro, G. A., Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy, Biotechnol. Bioeng., 66, 189-194 (1999)
Morγo, A., Maia, C. I., Fonseca, M. M. R. da, Vasconcelos, J. M. T., Alves, S. S., Effect of antifoam addition on gas-liquid mass transfer in stirred fermenters, Bioprocess Eng., 20, 165-172 (1999)
Morna-Gomes, A., Serralheiro, M. L. M., Fevereiro, P. S., Aires-Barros, M. R., Application of factorial design to the optimization of peroxidase activity in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane, Appl. Biochem. Biotechnol., 82, 27-36 (1999)
Noites, I. S., O΄Kennedy, R. D., Levy, M. S., Abidi, N., Keshavarz-Moore, E., Rapid quantitation and monitoring of plasmid DNA using an ultrasensitive DNA-binding dye, Biotechnol. Bioeng., 66, 195-201 (1999)
Prazeres, D. M. F., Ferreira, G. N. M., Monteiro, G. A., Cooney, C. L., Cabral, J. M. S., Large scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks, Trends Biotechnol., 17, 169-174 (1999)
Queiroz, J. A., Garcia, F. A. P., Cabral, J. M. S., Purification of a Chromobacterium viscosum lipase by hydrophobic interaction chromatography, Isolation and Purification, 3, 1-7 (1999)
Serralheiro, M. L. M., Cabral, J. M. S., Thermal stability of a-chymotrypsin, native and chemically modified, inside reverse micelles during peptide synthesis, Biocatal. Biotrans., 17, 3-19 (1999)
Serralheiro, M. L. M., Prazeres, D. M. F., Cabral, J. M. S., Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor, Enzyme Microb. Technol., 24, 507-513 (1999)
Serralheiro, M. L. M., Cabral, J. M. S., Irreversible thermoinactivation of a-chymotrypsin in buffer and water miscible organic solvent. Comparison with a reverse micellar system, J. Mol. Catal. B: Enzym., 7, 191-205 (1999)
Almeida, M. C., Venβncio, A., Teixeira, J. A., Aires-Barros, M. R., Cutinase purification on poly(ethylene glycol)-hydroxypropyl starch aqueous two-phase systems, J. Chromat. B, 711, 151-159 (1998)
Carvalho, C. M. L., Aires-Barros, M. R., Cabral, J. M. S., Cutinase structure, function and biocatalysis applications, Electronic J. Biotechnol., 1, (1998)
Carvalho, C. M. L., Cabral, J. M. S., Aires-Barros, M. R., Kinetics and modelling of transesterification reactions catalysed by cutinase in AOT reversed micelles, J. Mol. Catal. B: Enzym., 5, 361-365 (1998)
Castellar, M. R., Aires-Barros, M. R., Cabral, J. M. S., Iborra, J. L., Effect of zeolite addition on ethanol production from glucose by Saccharomyces bayanus, J. Chem. Technol. Biotechnol., 73, 377-384 (1998)
Cunha, M. T., Tjerneld, F., Cabral, J. M. S., Aires-Barros, M. R., Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution, J. Chromat. B, 711, 53-60 (1998)
Diogo, M. M., Cabral, J. M. S., Queiroz, J. A., Preliminary study on hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilized on Sepharose, J. Chromat. A, 796, 177-180 (1998)
Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Influence of some operational parameters on the bioconversion of sitosterol with immobilized whole cells in organic medium, J. Mol. Catal. B: Enzym., 5, 307-310 (1998)
Ferreira, B. S., Fernandes, H. L., Reis, A., Mateus, M., Microporous hollow fibres for carbon dioxide absorption: mass transfer model fitting and the supplying of carbon dioxide to microalgal cultures, J. Chem. Technol. Biotechnol., 71, 61-70 (1998)
Ferreira, B. S., van Keulen, F., Fonseca, M. M. R. da, Novel calibration method for mass spectrometers for on-line gas analysis: Set-up for the monitoring of a bacterial fermentation, Bioprocess Eng., 19, 289-296 (1998)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., Purification of supercoiled plasmid DNA using chromatographic processes, J. Molecular Recognition, 11, 250-251 (1998)
Fonseca, L. J. P., Patrνcio, I. M., Cabral, J. M. S., Preliminary studies on continuous recovery and purification of the penicillin acylase under pseudo-affinity conditions using phenyl-Sepharose gel, J. Molecular Recognition, 11, 252-254 (1998)
Fontes, N., Almeida, M. C., Peres, C., Garcia, S., Grave, J., Aires-Barros, M. R., Soares, C. M., Cabral, J. M. S., Maycock, C. D., Barreiros, S., Cutinase activity and enantioselectivity in supercritical fluids, Ind. Eng. Chem. Res., 37, 3189-3194 (1998)
Giordano, R. C., Giordano, R. L. C., Prazeres, D. M. F., Cooney, C. L., Analysis of a Taylor-Poiseuille vortex flow reactor - I: Flow patterns and mass transfer characteristics, Chem. Eng. Sci, 53, 3635-3652 (1998)
Gonηalves, A. P. V., Cabral, J. M. S., Aires-Barros, M. R., Analysis of a BSTR for triglyceride hydrolysis with an immobilised cutinase, J. Mol. Catal. B: Enzym., 5, 35-38 (1998)
Leσn, R., Fernandes, P., Pinheiro, H. M., Cabral, J. M. S., Whole-cell biocatalysis in organic media, Enzyme Microb. Technol., 23, 483-500 (1998)
Lopes, J. P., Menezes, J. C., Faster development of fermentation processes. Early stages process diagnostics, AIChE Symp. Series, 94, 391-396 (1998)
Marcos, J. C., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Variation of penicillin acylase partition coefficient with phase volume ratio in poly(ethylene glycol)-sodium citrate aqueous two-phase systems, J. Chromat. B, 711, 295-299 (1998)
Melo, E. P., Carvalho, C. M. L., Aires-Barros, M. R., Costa, S. M. B., Cabral, J. M. S., Deactivation and conformational changes of cutinase in reverse micelles, Biotechnol. Bioeng., 58, 380-386 (1998)
Oliveira, A. C., Rosa, M. F., Cabral, J. M. S., Aires-Barros, M. R., Improvement of alcoholic fermentations by simultaneous extraction and enzymatic esterification of ethanol, J. Mol. Catal. B: Enzym., 5, 29-33 (1998)
Prazeres, D. M. F., Schluep, T., Cooney, C. L., Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography, J. Chromat. A, 806, 31-45 (1998)
Qin, Y., Cabral, J. M. S., Influence of Nernst-Planck diffusion on hollow-fiber mass-transfer processes, AIChE J., 44, 1529-1541 (1998)
Qin, Y., Cabral, J. M. S., Lumen mass transfer in hollow-fiber membrane processes with nonlinear boundary conditions, AIChE J., 44, 836-848 (1998)
Qin, Y., Cabral, J. M. S., Theoretical analysis on the design of hollow fiber modules and modules cascades for the separation of diluted species, J. Memb. Sci., 143, 197-205 (1998)
Serra, M. C. C., Fonseca, M. M. R. da, Calado, J. C. G., Palavra, A. M. F., Solubility of propene in water and in a mineral medium for the cultivation of a Xanthobacter strain, J. Solution Chemistry, 27, 455-461 (1998)
Serralha, F. N., Lopes, J. M., Lemos, F., Prazeres, D. M. F., Aires-Barros, M. R., Cabral, J. M. S., Ramτa Ribeiro, F., Zeolites as supports for an enzymatic alcoholysis reaction, J. Mol. Catal. B: Enzym., 4, 303-311 (1998)
Taipa, M. A., Kaul, R., Mattiasson, B., Cabral, J. M. S., Preliminary studies on the purification of a monoclonal antibody by affinity precipitation with Eudragit S-100, J. Molecular Recognition, 11, 240-242 (1998)
van Keulen, F., Correia, C. N., Fonseca, M. M. R. da, Solvent selection for the biotransformation of terpenes by Pseudomonas putida, J. Mol. Catal. B: Enzym., 5, 295-299 (1998)
Vasconcelos, J. M. T., Alves, S. S., Nienow, A. W., Bujalski, W., Scale-up of mixing in gassed multi-turbine agitated vessels, Can. J. Chem. Eng., 76, 398-404 (1998)
Alves, S. S., Vasconcelos, J. M. T., Barata, J., Alternative compartment models of mixing in tall tanks agitated by multi-Rushton turbines, Chem. Eng. Res. Des. (Part A), 75, 334-338 (1997)
Cabral, J. M. S., Aires-Barros, M. R., Pinheiro, H., Prazeres, D. M. F., Biotransformation in organic media by enzymes and whole cells, J. Biotechnol., 59, 133-143 (1997)
Carvalho, C. M. L., Serralheiro, M. L. M., Cabral, J. M. S., Aires-Barros, M. R., Application of factorial design to the study of transesterification reactions using cutinase in AOT-reversed micelles, Enzyme Microb. Technol., 21, 117-123 (1997)
Cunha, M. T., Cabral, J. M. S., Aires-Barros, M. R., Quantification of phase composition in aqueous two-phase systems of Breox/phosphate and Breox/Reppal PES 100 by isocratic HPLC, Biotechnol. Tech., 11, 351-353 (1997)
Esquνvel, M. M., Bandarra, N. M., Fontan, I., Bernardo-Gil, M. G., Batista, I., Nunes, M. L., Empis, J. A., Supercritical carbon dioxide extraction of sardine Sardina pilchardus oil, Lebensmittel-Wissenschaft und Technologie, 30, 715-720 (1997)
Feliciano, A. S., Cabral, J. M. S., Prazeres, D. M. F., Solubility studies and synthesis of AcPheLeuNH2 in reversed micellar systems, Biocatal. Biotrans., 14, 219-234 (1997)
Feliciano, A. S., Cabral, J. M. S., Prazeres, D. M. F., Quantitative structure activity relationships in the synthesis of AcXYNH2 dipeptides by a-chymotrypsin in reversed micelles, Enzyme Microb. Technol., 21, 284-290 (1997)
Ferreira, G. N. M., Cabral, J. M. S., Prazeres, D. M. F., A comparison of gel filtration chromatographic supports for plasmid purification, Biotechnol. Tech., 11, 417-420 (1997)
Gonηalves, A. P. V., Lopes, J. M., Lemos, F., Ramτa-Ribeiro, F., Prazeres, D. M. F., Cabral, J. M. S., Aires-Barros, M. R., Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi, Enzyme Microb. Technol., 20, 93-101 (1997)
Krieger, N., Taipa, M. A., Aires-Barros, M. R., Melo, E. H. M., Lima-Filho, J. L., Cabral, J. M. S., Purification of Penicillium citrinum lipase using AOT reversed micelles, J. Chem. Technol. Biotechnol., 69, 77-85 (1997)
Krieger, N., Taipa, M. A., Melo, E. H. M., Lima-Filho, J. L., Aires-Barros, M. R., Cabral, J. M. S., Kinetic characterization of the Penicillium citrinum lipase in AOT/isooctane-reversed micelles, Appl. Biochem. Biotechnol., 67, 87-95 (1997)
Melo, E. P., Aires-Barros, M. R., Costa, S. M. B., Cabral, J. M. S., Thermal unfolding of proteins at high pH range studied by UV absorbance, J. Biochem. Biophys. Methods, 34, 45-59 (1997)
Molinari, F., Aragozzini, F., Cabral, J. M. S., Prazeres, D. M. F., Continuous production of isovaleraldehyde through extractive bioconversion in a hollow-fiber membrane bioreactor, Enzyme Microb. Technol., 20, 604-611 (1997)
Oliveira, A. C., Rosa, M. F., Cabral, J. M. S., Aires-Barros, M. R., Immobilization of Saccharomyces cerevisiae cells and Rhizomucor miehei lipase for the production and extractive biocatalysis of ethanol, Bioprocess Eng., 16, 349-353 (1997)
Pissarra, P. N., Nielsen, J., Thermodynamics of metabolic pathways for penicillin production: Analysis of thermodynamic feasibility and free energy changes during fed-batch cultivation, Biotechnol. Prog., 13, 156-165 (1997)
Porto, A. L. F., Lima-Filho, J. L., Aires-Barros, M. R., Cabral, J. M. S., Tambourgi, E. B., Extraction of recombinant cytochrome b5 from disrupted Escherichia coli cells with an aqueous two-phase system in a continuous perforated rotating disc contactor, Biotechnol. Tech., 11, 641-643 (1997)
Prazeres, D. M. F., A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography, Chem. Eng. Sci., 52 (6), 953-960 (1997)
Qin, Y., Cabral, J. M. S., Lumen mass transfer in hollow-fiber membrane processes with constant wall resistances, AIChE J., 43, 1975-1988 (1997)
Sarmento, M. J., Pires, M. J., Cabral, J. M. S., Aires-Barros, M. R., Liquid-liquid extraction of a recombinant protein, cytochrome b5, from an impure extract using aqueous two-phase systems, Bioprocess Eng., 16, 295-297 (1997)
Sebastiγo, M. J., Martel, P., Baptista, A., Petersen, S. B., Cabral, J. M. S., Aires-Barros, M. R., Predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems, Biotechnol. Bioeng., 56, 248-257 (1997)
Vasconcelos, J. M. T., Nienow, A. W., Martin, T., Alves, S. S., McFarlane, C. M., Alternative ways of applying the hydrogen peroxide steady state method of KLa measurement, Chem. Eng. Res. Des. (Part A), 75, 467-472 (1997)
Alves, S. S., Vasconcelos, J. M. T., Optimisation of agitation and aeration in fermenters, Bioprocess Eng., 14, 119-123 (1996)
Belo, I., Santos, J. A. L., Cabral, J. M. S., Mota, M., Optimization study of Escherichia coli TB1 cell disruption for cytochrome b5 recovery in small-scale bead mill, Biotechnol. Prog., 12, 201-204 (1996)
Cabral, J. M. S., Aires-Barros, M. R., Biocatalysis in organic media by cutinase immobilized in reversed micelles and zeolites, Chimia, 50, 420-421 (1996)
Carneiro-da-Cunha, M. G., Melo, E. P., Cabral, J. M. S., Aires-Barros, M. R., Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles, Bioprocess Eng., 15, 151-157 (1996)
Carneiro-da-Cunha, M. G., Aires-Barros, M. R., Tambourgi, E. B., Cabral, J. M. S., Continuous extraction of a recombinant cutinase from Escherchia coli disrupted cells with reversed micelles using a perforated rotating disc contactor, Bioprocess Eng., 15, 253-256 (1996)
Castellar, M. R., Taipa, M. A., Cabral, J. M. S., Kinetic and stability characterization of Chromobacterium viscosum lipase and its comparison with Pseudomonas glumae lipase, Appl. Biochem. Biotechnol., 61, 299-314 (1996)
Costa, M. E. L., van Gulik, W. M., ten Hoopen, H. J. G., Pais, M. S. S., Cabral, J. M. S., Protease and phenol production of Cynara cardunculus L. cell suspension in a chemostat, Enzyme Microb. Technol., 19, 493-500 (1996)
Cunnah, P., Aires-Barros, M. R., Cabral, J. M. S., Esterification and transesterification catalysed by cutinase in reverse micelles of CTAB for the synthesis of short chain esters, Biocatal. Biotrans., 14, 125-146 (1996)
Fonseca, L. P., Cabral, J. M. S., Evaluation of affinity and pseudo-affinity adsorption processes for penicillin acylase purification, Bioseparation, 6, 293-302 (1996)
Gonηalves, A. P. V., Cabral, J. M. S., Aires-Barros M. R., Immobilization of a recombinant cutinase by entrapment and by covalent binding: kinetic and stability studies, Appl. Biochem. Biotechnol., 60, 217-228 (1996)
Gonηalves, A. P. V., Lopes, J. M., Lemos, F., Ribeiro, F. R., Prazeres, D. M. F., Cabral, J. M. S., Aires-Barros, M. R., Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites, J. Mol. Catal. B: Enzym., 1, 53-60 (1996)
Lima-Costa, M. E., van Gulik, W. M., ten Hoopen, H. J. G., Pais, M. S. S., Cabral, J. M. S., Protease and phenol production of Cynara cardunculus L. cell suspension in a chemostat, Enzyme Microb. Technol., 19, 493-500 (1996)
Mateus, D. M. R., Alves, S. S., Fonseca, M. M. R. da, Model for the production of L-tryptophan from L-serine and indole by immobilized cells in a three-phase liquid-impelled loop reactor, Bioprocess Eng., 14, 151-158 (1996)
Mateus, D. M. R., Fonseca, M. M. R. da, Alves, S. S., Hydrodynamics and mass transfer in a liquid-impelled loop reactor, Ind. Eng. Chem. Res., 35, 4559-4565 (1996)
Melo, E. P., Costa, S. M. B., Cabral, J. M. S., Denaturation of a recombinant cutinase from Fusarium solani pisi in AOT - iso-octane reverse micelles: a steady-state fluorescence study, Photochem. Photobiol., 63, 169-175 (1996)
Morgado, M. A. P., Cabral, J. M. S., Prazeres, D. M. F., Phospholipase A2-catalysed hydrolysis of lecithin in a continuous reversed-micellar membrane bioreactor, J. A. O. C. S., 73, 337-346 (1996)
Pinto-Sousa, A. M., Cabral, J. M. S., Aires-Barros, M. R., Stability of a Fusarium solani pisi recombinant cutinase in phosphatidylcholine reversed micelles, Biotechnol Lett., 18, 583-586 (1996)
Pires, M. J., Aires-Barros, M. R., Cabral, J. M. S., Liquid-liquid extraction of proteins with reversed micelles, Biotechnol. Prog., 12, 290-301 (1996)
Pissarra, P. N., Nielsen, J., Bazin, M. J., Pathway kinetics and metabolic control analysis of a high-yielding strain of Penicillium chrysogenum during fed batch cultivations, Biotechnol. Bioeng., 51, 168-176 (1996)
Prazeres, D. M. F., Optimal design of a number of membrane reactors in series using Michaelis-Menten one-substrate reversible kinetics, Biotechnol. Tech., 10, 313-318 (1996)
Qin, Y., Cabral, J. M. S., Comparison between supported gas membrane process and supported liquid membrane process for the separation of NH3 from aqueous media containing NH3 and CO2, J. Chem. Technol. Biotechnol., 67, 323-328 (1996)
Qin, Y., Cabral, J. M. S., Hollow fiber supported liquid membrane process for the selective separation of NH3 from aqueous media containing NH3 and CO2, J. Chem. Technol. Biotechnol., 65, 137-148 (1996)
Qin, Y., Cabral, J. M. S., Wang, S.C., Hollow-fiber gas-membrane process for removal of NH3 from solution of NH3 and CO2, AIChE J., 42, 1945-1956 (1996)
Queiroz, J. A., Garcia, F. A. P., Cabral, J. M. S., Hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polyethylene glycol immobilized on Sepharose, J. Chromat. A., 734, 213-219 (1996)
Ramτa-Ribeiro, F., Gonηalves, A. P. V., Lemos, F., Prazeres, D. M. F., Cabral, J. M. S., Aires-Barros, M. R., Lopes, J. M., Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites, J. Mol. Catal. B: Enzym. , 1, 53-60 (1996)
Rodrigues, M. F., Gil, M. G. B., Vapor-liquid equilibrium data of a-pinene + b-pinene + limonene at 80 kPa and 101 kPa, J. Chem. Eng. Data, 41, 581-585 (1996)
Santos, J. A. L., Belo, I., Mota, M., Cabral, J. M. S., Freeze/thawing and sonication of Escherichia coli TB1 cells for cytochrome b5 recovery, Bioseparation, 6, 81-89 (1996)
Sebastiγo, M. J., Cabral, J. M. S., Aires-Barros, M. R., Improved purification protocol of a Fusarium solani recombinant cutinase by phase partitioning in aqueous two-phase systems of polyethylene glycol and phosphate, Enzyme Microb. Technol., 18, 251-260 (1996)
Vasconcelos, J. M. T., Barata, J. M., Alves, S. S., Transitional mixing in multiple-turbine agitated tanks, Chem. Eng. J., 63, 53-58 (1996)
Alves, J. R. S., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Separation and purification of penicillin acylase from Escherichia coli using AOT reverse micelles, Biotechnol. Tech., 9, 265-270 (1995)
Alves, J. R. S., Fonseca, L. P., Ramalho, M. T., Cabral, J. M. S., Assay of penicilin acylase in organic media, Biotechnol. Tech., 9, 729-730 (1995)
Alves, S. S., Vasconcelos, J. M. T., Mixing in gas-liquid contactors agitated by multiple turbines in the flooding regime, Chem. Eng. Sci., 50, 2355-2357 (1995)
Fernandes, P., Cabral, J. M. S., Pinheiro, H. M., Bioconversion of a hydrocortisone derivative in an organic-aqueous two-liquid phase system, Enzyme Microb. Technol., 17, 163-167 (1995)
Ferreira-Dias, S., Fonseca, M. M. R. da, Glycerolysis of olive oil: batch operational stability of Candida rugosa lipase immobilized in hydrophilic polyurethane foams, Bioprocess Eng., 13, 311-315 (1995)
Ferreira-Dias, S., Fonseca, M. M. R. da, Production of monoglycerides by glycerolysis of olive oil with immobilized lipases: effect of the water activity, Bioprocess Eng., 12, 327-337 (1995)
Ferreira-Dias, S., Fonseca, M. M. R. da, The effect of substrate hydrophobicity on the kinetic behaviour of immobilized Candida rugosa lipase, Biocatal. Biotrans., 13, 99-110 (1995)
Kennedy, J. F., Cabral, J. M. S., Immobilisation of biocatalysts by metal-link chelation processes, Artif. Cells, Blood Subs. Immob. Biotech., 23, 231-252 (1995)
Mateus, M., Cabral, J. M. S., Modeling membrane filtration of protein and cell-suspensions in a vortex flow filtration system, AIChE J., 41, 764-772 (1995)
Melo, E. P., Ivanova, M. G., Aires-Barros, M. R., Cabral, J. M. S., Verger, R., Glyceride synthesis catalyzed by cutinase using the monomolecular film technique, Biochem., 34, 1615-1621 (1995)
Melo, E. P., Aires-Barros, M. R., Cabral, J. M. S., Triglyceride hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-isooctane reversed micelles, Appl. Biochem. Biotechnol., 50, 45-56 (1995)
Melo, E. P., Costa, S. M. B., Cabral, J. M. S., Structural effect of reversed micelles of AOT over a recombinant cutinase from Fusarium solani pisi. A steady-state fluorescence study, Ann. N. Y. Acad. Sci., 750, 85-88 (1995)
Melo, N. S., Cabral, J. M. S., Fevereiro, M. P., Extracellular peroxidases from cell suspension cultures of Vaccinium myrtillus. Purification and characterization of two cationic enzymes, Plant Sci., 106, 177-184 (1995)
Mendes, R. L., Coelho, J. A. P., Fernandes, H. L., Marrucho, I. J., Cabral, J. M. S., Novais, J. M., Palavra, A. M. F., Applications of supercritical CO2 extraction to microalgae and plants, J. Chem. Technol. Biotechnol., 62, 53-59 (1995)
Mendes, R. L., Fernandes, H. L., Coelho, J. P., Reis, E. C., Cabral, J. M. S., Novais, J. M., Palavra, A. F., Supercritical CO2 extraction of carotenoids and other lipids from Chlorella vulgaris, Food Chemistry, 53, 99-103 (1995)
Morgado, M. P., Cabral, J. M. S., Prazeres, D. M. F., Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulfosuccinate, J. Chem. Technol. Biotechnol., 63, 181-189 (1995)
Nabais, A. M. A., Cardoso, J. P., Ultrafiltration of fermented broths and solvent extraction of antibiotics, Bioprocess Eng., 13, 215-221 (1995)
Nabais, A. M. A., Fonseca, M. M. R. da, The effect of solid medium composition on growth and sporulation of Streptomyces clavuligerus: spore viability during storage at +4°C, Biotechnol. Tech., 9, 361-364 (1995)
Prazeres, D. M. F., Concentration of BSA using a superabsorbent polymer: process evaluation, J. Biotechnol., 39, 157-164 (1995)
Prazeres, D. M. F., Optimal design of N CSTRs in series using Michaelis-Menten one-substrate reversible reaction, Biocatal. Biotrans., 13, 43-52 (1995)
Prazeres, D. M. F., Optimal design of N membrane reactors in series using Michaelis-Menten kinetics, Can. J. Chem. Eng., 73, 931-934 (1995)
Queiroz, J. A., Garcia, F. A. P., Cabral, J. M. S., Partitioning of Chromobacterium viscosum lipases in aqueous two-phase systems, Bioseparation, 5, 307-311 (1995)
Queiroz, J. A., Garcia, F. A. P., Cabral, J. M. S., Hydrophobic interaction chromatography of Chromobacterium viscosum lipase, J. Chromat. A, 707, 137-142 (1995)
Ribeiro, M. A., Gil, M. G. B., Solubilities of triolein in supercritical CO2, J. Chem Eng. Data, 40, 1188-1192 (1995)
Ribeiro, M. H. L., Prazeres, D. M. F., Cabral, J. M. S., Fonseca, M. M. R. da, Adsorption studies for the separation of L-tryptophan from L-serine and indole in a bioconversion medium, Bioprocess Eng., 12, 95-102 (1995)
Rodrigues, M. F., Gil, M. G. B., Vapour-liquid equilibrium of binary mixtures of limonene with a-pinene and b-pinene at reduced pressures, J. Chem. Eng. Data, 40, 1193-1195 (1995)
Sebastiγo, M. J., Cabral, J. M. S., Aires-Barros, M. R., Simultaneous quantification of polymer and salt in polyethylene glycol/phosphate aqueous two-phase systems by HPLC, Biotechnol. Tech., 9, 503-508 (1995)
Setti, L., Fevereiro, M. P., Melo, E. P., Pifferi, P. G., Cabral, J. M. S., Aires-Barros, M. R., Superactivity of peroxidase solubilized in reverse micellar systems, Appl. Biochem. Biotechnol., 55, 207-218 (1995)
Taipa, M. A., Liebeton, K., Costa, J. V., Cabral, J. M. S., Jaeger, K.- E., Lipase from Chromobacterium viscosum: Biochemical characterization indicating homology to the lipase from Pseudomonas glumae, Biochim. Biophys. Acta, 1256, 396-402 (1995)
Vasconcelos, J. M. T., Alves, S. S., Barata, J. M., Mixing in gas-liquid contactors agitated by multiple turbines, Chem. Eng. Sci., 50, 2343-2354 (1995)
Caldeira, J. C. G., Cabral, J. M. S., Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells, Bioseparation, 4, 271-278 (1994)
Carneiro-da-Cunha, M. G., Cabral, J. M. S., Aires-Barros, M. R., Studies on the extraction and back-extraction of a recombinant cutinase in a reversed micelles extraction process, Bioprocess Eng., 11, 203-208 (1994)
Carneiro-da-Cunha, M. G., Aires-Barros, M. R., Tambourgi, E. B., Cabral, J. M. S., Recovery of a recombinant cutinase with reversed micelles in a continuous perforated rotating disc contactor, Biotechnol. Tech., 8, 413-418 (1994)
Dias, A. C. P., Cabral, J. M. S., Pinheiro, H. M., Sterol side-chain cleavage with immobilized Mycobacterium cells in water-immiscible organic solvents, Enzyme Microb. Technol., 16, 708-714 (1994)
Fonseca, L. P., Caldeira, J. C. G., Cabral, J. M. S., Improvement in the polyethylene glycol-cibacron blue purification method, J. Chromat. A., 668, 61-64 (1994)
Mendes, R. L., Fernandes, H. L., Coelho, J. A. P., Cabral, J. M. S., Palavra, A. M. F., Novais, J. M., Supercritical carbon dioxide extraction of hydrocarbons from the microalga Botryococcus braunii, J. Appl. Phycol., 6, 289-293 (1994)
Menezes, J. C., Alves, S. S., Lemos, J. M., Azevedo, S. F., Mathematical modelling of industrial pilot-plant penicillin-G fed-batch fermentations, J. Chem. Technol. Biotechnol., 61, 123-138 (1994)
Pinto-Sousa, A. M. C., Cabral, J. M. S., Aires-Barros, M. R., Ester synthesis by a recombinant cutinase in reversed micelles of a natural phospholipid, Biocatalysis, 9, 169-179 (1994)
Pires, M. J., Cabral, J. M. S., Thermal stability of a Desulfovibrio gigas periplasmic hydrogenase in reverse micelles, Appl. Biochem. Biotechnol., 48, 137-147 (1994)
Pires, M. J. L., Cabral, J. M. S., Liquid-liquid extraction of a recombinant protein with reverse micelles, J. Chem. Technol. Biotechnol., 61, 219-224 (1994)
Pires, M. J. L., Martel, P., Baptista, A., Petersen, S. B., Willson, R. C., Cabral, J. M. S., Improving protein extraction yield in reversed micellar systems through surface charge engineering, Biotechnol. Bioeng., 44, 773-780 (1994)
Prazeres, D. M. F., Garcia, F. A. P., Cabral, J. M. S., Continuous lipolysis in a reversed micellar membrane bioreactor, Bioprocess Eng., 10, 21-27 (1994)
Prazeres, D. M. F., Cabral, J. M. S., Enzymatic membrane bioreactors and their applications, Enzyme Microb. Technol., 16, 738-750 (1994)
Qin, Y., Cabral, J. M. S., Kinetic studies of the urease-catalyzed hydrolysis of urea in a buffer-free system, Appl. Biochem. Biotechnol., 49, 217-240 (1994)
Sarmento, M. J., Pires, M. J. L., Cabral, J. M. S., Aires-Barros, M. R., Liquid-liquid extraction of a recombinant protein, cytochrome b5, with aqueous-two phase systems of polyethylene glycol and potassium phosphate salts, J. Chromat. A, 668, 117-120 (1994)
Sebastiγo, M. J., Cabral, J. M. S., Aires-Barros, M. R., Partitioning of recombinant Fusarium solani pisi cutinase in polyethylene glycol-aqueous salt solution two-phase systems, J. Chromat. A, 668, 139-144 (1994)
Serralheiro, M. L. M., Prazeres, D. M. F., Cabral, J. M. S., Dipeptide synthesis and separation in a reversed micellar membrane reactor, Enzyme Microb. Technol., 16, 1064-1073 (1994)
Serralheiro, M. L., Cabral, J. M. S., Synthesis of AcPheLeuNH2 by a-chymotrypsin in TTAB reversed micelles: application of response surface methodology to the optimization of the system, Biotechnol. Bioeng., 43, 1031-1042 (1994)
Taipa, M. A., Moura-Pinto, P., Cabral, J. M. S., Identification and characterization of Chromobacterium viscosum lipase, Biotechnol. Tech., 8, 27-32 (1994)
van Keulen, F., Fonseca, M. M. R. da, Batch cultivation of Xanthobacter Py2 on 1-pentene: growth yield and cell activity for the degradation of 1,2-epoxyhexane, Biotechnol. Lett., 16, 989-994 (1994)
Vicente, L. C., Aires-Barros, M. R., Empis, J. M. A., Stability and proteolytic activity of papain in reverse micellar and aqueous media: a kinetic and spectroscopic study, J. Chem. Technol. Biotechnol., 60, 291-297 (1994)
Videira, M. M., Aires-Barros, M. R., Liquid-liquid extraction of clavulanic acid using an aqueous two phase system of polyethylene glycol and potassium phosphate, J. Chromat. A, 668, 237-240 (1994)