2012
de Barros, D.P.C., Azevedo, A.M., Cabral, J.M.S., Fonseca, L.P., “Optimization of flavor esters synthesis by fusarium solani pisi cutinase”, J. Food Biochem., 36(3), 275-284
Borlido, L., Azevedo, A.M., Sousa, A.G., Oliveira, P.H., Roque, A.C.A., Aires-Barros, M.R., “Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles”, J. Chromatogr. B, 903, 163-70
Madeira, P.P., Bessa, A., Alvares-Ribeiro, L., Aires-Barros, M.R., Reis, C. A., Rodrigues, A.E., Zaslavsky, B.Y., “Salt effects on solvent features of coexisting phases in aqueous polymer/polymer two-phase systems”, J. Chromatogr. A, 1229, 38-47
Rosa, P.A.J., Azevedo, A.M., Sommerfeld, S., Baecker, W., Aires-Barros, M.R., “Continuous aqueous two-phase extraction of human antibodies using a packed column”, J. Chromatogr. B, 880, 148-156
Silva, D.F.C., Azevedo, A.M., Fernandes, P., Chu, V., Conde, J.P., Aires-Barros, M.R., “Design of a microfluidic platform for monoclonal antibody extraction using an aqueous two-phase system”, J. Chromatogr. A, 1249, 1-7
2011
de Barros, D.P.C., Fernandes, P., Cabral, J.M.S., Fonseca, L.P., “Synthetic application and activity of cutinase in an aqueous, miniemulsion model system: Hexyl octanoate synthesis”, Catal. Today, 173, 95-102 (2011)
de Barros, D.P.C., Azevedo, A.M., Cabral, J.M.S., Fonseca, L.P., “Optimisation of flavour esters synthesis by Fusarium solani pisi cutinase”, J. Food Biochem., 58(2), 545-556 (2011)
Borlido, L., Azevedo, A.M., Roque, A.C.A., Aires-Barros, M.R., “Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions”, J. Chromatogr. A, 1218, 7821-7827 (2011)
Gomes, A.G., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads”, J. Chromatogr. A., 1218, 8629-8637 (2011)
Madeira, C., Loura, L.M.S., Aires-Barros, M.R., Prieto, M., “Fluorescence methods for lipoplex characterization”, Biochim. Biophys. Acta – Biomembr., 1808, 2694-2705(2011)
Nascimento, K.S., Yelo, S., Cavada, B.S., Azevedo, A.M., Aires-Barros, M.R., “Liquid-liquid equilibrium data for aqueous two-phase systems composed of ethylene oxide propylene oxide copolymers”, J. Chem. Eng. Data, 56, 190-194 (2011)
Rosa, P.A.J., Azevedo, A.M., Sommerfeld, S., Bäcker, W., Aires-Barros, M. R., "Aqueous two-phase extraction as a platform in the biomanufacturing industry: Economical and environmental sustainability", Biotechnol. Adv., 29, 559-567 (2011)
Sousa, A.G., Andrade, P.Z., Pirzgalska, R.M., Galhoz, T.M., Azevedo, A.M., da Silva, C.L., Aires-Barros, M.R., Cabral, J.M.S., “A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinty aqueous two-phase partitioning”, Biotechnol. Lett., 33, 2373-2377 (2011)
2010
Azevedo, A.M., Gomes, A.G., Borlido, L., Santos, I.S., Prazeres, D.M.F., Aires-Barros, M.R., “Capture of human monoclonal antibodies from a cell culture supernatant by phenyl boronate chromatography” J. Mol. Recognit., 23, 569-576 (2010)
x
Borlido, L., Azevedo, A.M., Aires-Barros, M.R., “Extraction of human IgG in thermo-responsive aqueous two-phase systems: Assessment of structural stability by circular dichroism”, Sep. Sci. Technol., 45, 2171-2179 (2010)
Nascimento, K.S., Azevedo, A.M., Cavada, B.S., Aires-Barros, M.R., “Partitioning of Canavalia brasiliensis Lectin in Polyethylene Glycol - Sodium Citrate Aqueous Two-Phase Systems” Sep. Sci. Technol., 45, 2180-2186 (2010)
Nascimento, K.S., Rosa, P.A.J., Nascimento, K.S., Cavada, B.S., Azevedo, A.M., Aires-Barros, M.R., “Partitioning of Canavalia brasiliensis Lectin in Polyethylene Glycol - Sodium Citrate Aqueous Two-Phase Systems” Sep. Purif. Technol., 75, 48-54 (2010)
Rosa, P .A. J., Ferreira, I. F., Azevedo, A. M., Aires-Barros, M. R., "Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals", J. Chromatogr. A, 1217, 2296-2305 (2010)
2009
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., “Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing”, Trends Biotecnhol., 27, 240-247 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., de Vries, J., Visser, T. J.,
Aires-Barros, M. R., "Downstream processing of human antibodies integrating an extraction
capture step and cation exchange chromatography", J. Chromatogr. B, 887,50-58 (2009)
Azevedo, A. M., Gomes, A. G., Rosa, P. A. J, Ferreira, I. F., Pisco, A. M. M. O., Aires-Barros, M. R., “Partitioning of human antibodies in polyethylene glycol - sodium citrate aqueous two-phase systems”, Sep. Purif. Tecnhol., 65, 14-21 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Pisco, A. M. M. O., de Vries, J., Korporaal, R., Visser, T. J., Aires-Barros, M. R., “Affinity-enhanced purification of human antibodies by aqueous two-phase extraction”, Sep. Purif. Tecnhol., 65, 31-39 (2009)
Baecker, W., sommerfeld, S., Mutter, M., Rosa, P.A.A.J., Aires-Barros, M.R.M.S., Azevedo, A.M.N.M.P., "Method for purifying therapeutic proteins", World Patent WO/209/112149 (2009)
Rosa, P .A. J., Azevedo, A. M., Ferreira, I. F., Sommerfeld, S., Bäcker, W., Aires-Barros, M. R., "Downstream processing of antibodies:
Single-stage versus multi-stage aqueous two-phase extraction", J. Chromatogr. A, 1216, 8741-8749 (2009)
Rosa, P .A. J., Azevedo, A. M., Sommerfeld, S., Mutter, M., Aires-Barros, M. R., Bäcker, W., "Application of aqueous two-phase systems to antibody purification:
A multi-stage approach", J. Biotechnol.,139, 306-313 (2009)
Sousa, I. T., Ruiu, L., Lowe, C. R., Taipa, M. A., “Synthetic affinity ligands as a novel tool to improve protein stability”, J. Mol. Recognit., 22, 83-90 (2009)
2008
Azevedo, A. M., Rosa, P. A. J, Ferreira, I. F., Aires-Barros, M. R., "Integrated process
for the purification of antibodies combining aqueous two-phase extraction, hydrophobic
interaction chromatography and size-exclusion chromatography", J. Chromatogr. A, 1213,
154-161(2008)
Ferreira, I. F., Azevedo, A. M., Rosa, P. A. J., Aires-Barros, M. R., “Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems”, J. Chromatogr. A, 1195, 94-100 (2008)
Taipa, M. A., “Immunoassays: Biological tools for High Throughput Screening and characterisation of combinatorial libraries”, CCHTS, 11, 325-335 (2008)
2007
Azevedo, A. M. Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., “Optimisation of aqueous two-phase extraction of human antibodies”, J. Biotechnol., 132, 209-217 (2007)
Rosa, P. A. J., Azevedo, A. M., Aires-Barros, M. R., “Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies”, J. Chromatogr. A, 1141, 50-60 (2007)
Rosa, P. A. J., Azevedo, A. M., Ferreira, I. F., de Vries, J., Korporaal, R., Verhoef, H. J., Visser, T. J., Aires-Barros, M. R., “Affinity partitioning of human antibodies in aqueous two-phase systems”, J. Chromatogr. A, 1162, 103-113 (2007)
Roque, A. C. A., Silva, C. S. O., Taipa, M. A., “Affinity-based methodologies and ligands for antibody purification: Advances and Perspectives”, J. Chromatogr. A, 1160, 44-55 (2007)
