Selected Publications
Carvalho, J.A., Azzoni, A.R., Prazeres, D.M.F., Monteiro, G.A., “Comparative analysis of antigen-targeting sequences used in DNA vaccines”, Mol. Biotechnol. 47(1), 94-94 (2011)
Gomes, A.G., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads”, J. Chromatogr. A. 1218, 8629-8637 (2011)
Gonçalves, G.A.L., Bower, D.M., Prazeres, D.M.F., Monteiro, G.A., Prather, K.L.J., “Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing”, Biotechnol. J. 7(2), 166-311 (2011)
Guerrero-German, P., Montesinos-Cisneros, R.M., Prazeres, D.M.F., Tejeda-Mansir, A., “Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography”, Biotechnol. Appl. Biochem. 58(1), 68-74 (2011)
Joskowiak, A., Santos, M.S., Prazeres, D.M.F., Chu, V.; Conde, J.P., “Integration of thin film amorphous silicon photodetector with lab-on-chip for monitoring protein fluorescence in solution and in live microbial cells, Sens. Actuators B 156(2), 662-667 (2011)
Lança, A.S.C., de Sousa, K.F., Atouguia, J., Prazeres, D.M.F., Monteiro, G.A., Silva, M.S., “Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis”, Exp. Parasitol. 127(1), 18-24 (2011)
Martins, D.C., Prazeres, D.M.F., Chu, V., Conde, J.P., “Label-free detection of DNA immobilization and hybridization by streaming current measurements in microchannels”, App. Physics Lett. 99, 183702 (2011)
Novo, P., Prazeres, D.M.F., Chu, V., Conde, J.P., “Microspot-based ELISA in Microfluidics: Chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes”, Lab-on-a-Chip 11, 4063-4071 (2011)
Oliveira, P.H., Prather, K.L.J., Prazeres, D.M.F., Monteiro, G.A., “Mutation detection in plasmid-based biopharmaceuticals”, Biotechnol. J. 6(4), 378-391 (2011)
Pereira, A.T., Novo, P., Prazeres, D.M.F., Chu, V., Conde, J.P., “Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes”, Biomicrofluidics 5(1), 014102 (2011)
Santa, G.L.M., Bernardino, S.M.S.A., Magalhães, S., Mendes, V., Marques, M.P.C., Fonseca, L.P., Fernandes, P., “From inulin to fructose syrups using sol-gel immobilized inulinase”, Appl. Biochem. Biotechnol. 165(1), 1-12 (2011)
Botelho-Cunha, V.A., Mateus, M., Petrus, J.C.C., de Pinho, M.N., "Tailoring the enzymatic synthesis and nanofiltration fractionation of galacto-oligosaccharides", Biochem. Eng. J. 50, 29–36 (2010)
Carvalho, J., Azzoni, A.R., Prazeres, D.M.F., Monteiro, G.A., “Comparative analysis of antigen targeting sequences used in DNA vaccines”, Mol. Biotechnol. 44, 204-212 (2010)
Carvalho, J., Rodgers, J., Atouguia, J., Prazeres, D.M.F., Monteiro, G.A., “DNA vaccines: a rational design against parasitic diseases”, Expert Rev. Vaccines 9, 175-191 (2010)
Gomes, A.G., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption”, J. Chromatogr. A 1217, 2262-2266 (2010)
Martins, S.A.M., Prazeres, D.M.F., Fonseca, L.P., Monteiro, G.A., “Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification”, Biotechnol. Lett. 32, 229-234 (2010)
Raiado-Pereira, L., Prazeres, D.M.F., Mateus, M., "Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization", J. Sep. Sci. 33, 1175-1184 (2010)
Ribeiro, S.C., Monteiro, G. A., Prazeres, D. M. F., “Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin”, Appl. Microbiol. Biotechnol. 86, 671-679 (2010)
Cabeça, R., Rodrigues, M., Prazeres, D.M.F., Chu, V., Conde, J.P., “The Effect of the Shape of Single, Sub-ms Voltage Pulses on the Rates of Surface Immobilization and Hybridization of DNA”, Nanotechnology 20 (2009) 015503.
Gomes, G.A., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F., “Purification of Plasmid DNA Using Aqueous Two-Phase Systems with PEG 600 and Sodium Citrate/Ammonium Sulphate”., Sep. Purif. Technol. 65 (2009) 22-30.
Conde, J.P., Pimentel, A.C., Pereira, A.T., Gouvêa, A., Prazeres, D.M.F., Chu, V., “Detection of Molecular Tags with an Integrated Amorphous Silicon Photodetector for Biological Applications”, J. Non-Crystalline Sol. 354 (2008) 2594-2597.
Gonçalves, D., Prazeres, D.M.F., Chu, V., Conde, J.P., “Detection of DNA and Proteins Using Amorphous Silicon Ion-sensitive Thin-Film Field Effect Transistors” Biosensors and Bioelectronics 24 (2008) 545-551.
Martins, S., , Prazeres, D.M.F., Monteiro, G.A. “Chemiluminescent Bead-based Hybridization Assay for the Detection of Genomic DNA from E. coli in Purified Plasmid Samples”, Anal. Bioanal. Chem. 391 (2008) 2179-2187.
Oliveira, P.H., Lemos, F., Monteiro, G.A., Prazeres, D.M.F., “Recombination Frequency in Plasmid DNA Containing Direct Repeats - Predictive Correlation with Repeat and Intervening Sequence Length”, Plasmid 60 (2008) 159-165.
Pimentel, A.C, Prazeres, D.M.F., Chu, V., Conde, J.P. “Fluorescence Detection of DNA Using an Amorphous Silicon p-i-n Photodiode”, J. Applied Physics., 104 (2008) 054913.
Prazeres, D.M.F., “Prediction of Diffusion Coefficients of Plasmids”, Biotechnol. Bioeng. 99 (2008) 1040-1044.
Ribeiro, S.C., Oliveira, P.H., Prazeres, D.M.F., Monteiro, G.A, “High Frequency Plasmid Recombination Mediated by 28 bp Direct Repeats”, Molecular Biotechnol., 40 (2008) 252-260.
Sousa, F., Prazeres, D.M.F., Queiroz, J.A., “Affinity Chromatography Approaches for Overcoming the Challenges of Purifying Plasmid DNA” Trends Biotechnol., 26 (2008) 518-525.
Sousa, F., Prazeres, D.M.F., Queiroz, J.A., “Specific Recognition of Supercoiled Plasmid DNA by Arginine Affinity Chromatography” Anal. Biochem., 374 (2008) 432-434.
Azzonni, A.R., Ribeiro, S.C., Monteiro, G.A., Prazeres, D.M.F., “The Impact of Polyadenylation Signal on Plasmid Resistance and Transgene Expression”, J. Gene Medicine, 9 (2007) 392-402.
Carapuça, E., Azzoni, A.R., Prazeres, D.M.F., Monteiro, G.A., Mergulhão, F.J.M., “Time-Course Determination of Plasmid Content in Eukaryotic and Prokaryotic Cells Using Real-Time PCR”, Molecular Biotechnol., 37 (2007) 120-126.
Duarte, D., Fortes, A.G., Prazeres, D.M.F., Marcos, J.C., “Preparation of Plasmid DNA Polyplexes from Alkaline Lysates by a Two-Step Aqueous Two-phase Extraction Process”, J. Chromatog. A 1164 (2007) 105-112.
Freitas, S.S., Azzonni, A.R., Santos, J.A.L., Monteiro, G.A., Prazeres, D.M.F., “On the Stability of Plasmid Vectors During Cell Culture and Purification”, Molecular Biotechnol., 36 (2007) 151-158.
Henriques, A.M., Fevereiro, M., Prazeres, D.M.F., Monteiro, G.A, “Preliminary Studies on the Development of a Candidate DNA Vaccine Against Maedi-Visna Virus”, Veterinary Immunol. Immunopathol., 119 (2007) 222-232.
Pimentel, A.C, Pereira, A. T., Chu, V., Prazeres, D.M.F., Conde, J.P. “Detection of Chemiluminescence Using an Amorphous Silicon Photodiode”, IEEE Sensors Lett., 7 (2007) 415-416.
Sousa, F., Prazeres, D.M.F., Queiroz, J.A., “Circular Dichroism Investigation of the Effect of Plasmid DNA Structure on Retention in Histidine Chromatography”, Arch. Biochem. Biophys., 467 (2007) 154-162.
Sousa, F., Prazeres, D.M.F., Queiroz, J.A., “Dynamic Binding Capacity of Plasmid DNA in Histidine-agarose Chromatography”, Biomedical Chromatog., 21 (2007) 993-998.
Passarinha, L., Diogo, M.M., Queiroz, J.A., Monteiro, G.A., Fonseca, L.P., Prazeres, D.M.F., “Production of Plasmid DNA by Escherichia coli Culture Under Non-Selective Conditions”, J. Microbiol. Biotechnol., 16 (2006) 20-24.
Gonçalves, D.I., Prazeres, D.M.F., Chu, V., Conde, J.P., “Label-Free Electronic Detection of Biomolecules Using a-Si:H Field Effect Devices”, J. Non-Crystalline Sol. 352 (2006) 2007-2010.
Adrega, T., Prazeres, D.M.F., Chu, V., Conde, J.P., “Thin-Film Silicon MEMS DNA Sensors”, J. Non-Crystalline Sol. 352 (2006) 1999-2003.
Freitas, S.S., Santos, J.A.L., Prazeres, D.M.F., “Optimisation of Isopropanol and Ammonium Sulphate Precipitation Steps in the Purification of Plasmid DNA”, Biotechnol. Prog., 22 (2006) 1179-1186.
Sousa, F., Freitas, S.S., Azzonni, A., Prazeres, D.M.F., Queiroz, J.A., “Purification and Isolation of Supercoiled Plasmid DNA from Cell Lysates with a Single Histidine-Chromatography Step”, Biotechnol. App. Biochem., 45 (2006) 131-140.