2012
de Barros, D.P.C., Azevedo, A.M., Cabral, J.M.S., Fonseca, L.P., “Optimization of flavor esters synthesis by fusarium solani pisi cutinase”, J. Food Biochem., 36(3), 275-284
Caramujo, M.-J., de Carvalho, C.C.C.R., Silva, S.J., Carman, K.R., “Dietary Carotenoids Regulate Astaxanthin Content of Copepods and Modulate Their Susceptibility to UV Light and Copper Toxicity”, Marine Drugs, 10(5), 998-1018
Carvalho, T., Augusto, V., Brás, A.R., Lourenço, N.M.T., Afonso, C.A.M., Barreiros, S., Correia, N.T., Vidinha, P., Cabrita, E.J., Dias, C.J., Dionísio, M., Roling, B., “Understanding the ion jelly conductivity mechanism”, J. Phys. Chem. B, 116(9), 2664-2676
de Carvalho, C.C.C.R., “Biofilms: new ideas for an old problem”, Recent Patents on Biotechnology, 6(1)
de Carvalho, C.C.C.R., “Adaptation of Rhodococcus erythropolis cells for growth and bioremediation under extreme conditions”, Res. Microbiol., 163(2), 125-136
de Carvalho, C.C.C.R., Caramujo, M.J., “Tumour metastasis as an adaptation of tumour cells to fulfil their phosphorus requirements”, Medical Hypotheses, 78(5), 664-667
Freitas, P.P., Cardoso, F. A., Martins, V. C., Martins, S. A. M., Loureiro, J., Amaral, J., Chaves, R. C., Cardoso, S., Fonseca, L.P., Sebastiao, A.M., Pannetier-Lecoeur, M., Fermon, C., “Spintronic platforms for biomedical applications”, Lab on a Chip, 12(3), 546-557
Marques, M.P.C., Fernandes, P., Cabral, J.M.S., Znidarsic-Plazl, P., Plaz, l., “Continuous steroid biotransformations in microchannel reactors”, New Biotechnol, 29(2), 227-234
Marques, M.P.C., Walshe, K., Doyle, S., Fernandes, P., de Carvalho, C.C.C.R., “Anchoring high-throughput screening methods to scaleup bioproduction of siderophores”, Process Biochem., 47(3), 416-421
Silva, D.F.C., Azevedo, A.M., Fernandes, P., Chu, V., Conde, J.P., Aires-Barros, M.R., “Design of a microfluidic platform for monoclonal antibody extraction using an aqueous two-phase system”, J. Chromatogr. A, 1249, 1-7
2011
Bernardino, S., Estrela, N., Ochoa-Mendes, V., Fernandes, P., Fonseca, L. P., "Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties", Journal of Sol-Gel Science and Technology, 58, 545-556 (2011)
Coutinho, C. P., de Carvalho, C. C. C. R., Madeira, A., Pinto-de-Oliveira, A., Sa-Correia, I., "Burkholderia cenocepacia Phenotypic Clonal Variation during a 3.5-Year Colonization in the Lungs of a Cystic Fibrosis Patient", Infection and Immunity, 79, 2950-2960 (2011)
de Carvalho, C. C. C. R., "Enzymatic and whole cell catalysis: Finding new strategies for old processes", Biotechnology Advances, 29, 75-83 (2011)
de Carvalho, C. C. C. R., Cruz, P. A., da Fonseca, M. M. R., Xavier-Filho, L., "Antimicrobial properties of the extract of Abelmoschus esculentus", Biotechnology and Bioprocess Engineering, xx, xx-xx (2011)
de Carvalho, C. C. C. R., Fonseca, M. M. R. d., "Biotransformations", Volume 2: Engineering Fundamentals in Biotechnology, Comprehensive Biotechnology, 2nd Edition, Collin Webb (Editor-in-Chief: Murray Moo-Young), Elsevier (2011)
Fernandes, P., Marques, M. P. C., Carvalho, F., de Carvalho , C. C. C. R., "Organic-solvent tolerant Gram-positive bacteria: applications and mechanisms of tolerance", Organic solvents: properties, toxicity and industrial effects, Carter, R. E., Nova Science Publishers (2011)
Marques, M. P. C., Lourenço, N. M. T., Fernandes, P., de Carvalho, C. C. C. R., "Green Solvents for Biocatalysis.", Green Solvents Properties and Applications in Chemistry, Mohammad, A., Inamuddin, M. P., Springer (2011)
Tyagi, M., da Fonseca, M. M. R., de Carvalho, C. C. C. R., "Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes", Biodegradation, 22, 231-241 (2011)
2010
Bernardino, S., Fernandes, P., Fonseca, L. P., "Improved Specific Productivity in Cephalexin Synthesis by Immobilized PGA in Silica Magnetic Micro-Particles", Biotechnology and Bioengineering, 107, 753-762 (2010)
de Barros, D. P. C., Fernandes, P., Cabral, J. M. S., Fonseca, L. P., "Operational stability of cutinase in organic solvent system: model esterification of alkyl esters", Journal of Chemical Technology and Biotechnology, 85, 1553-1560 (2010)
de Carvalho, C. C. C. R., "Adaptation of Rhodococcus to organic solvents", Microbiology Monographs, Vol. 16 Hector M. Alvarez (Alexander Steinbüchel, S. E., Springer Verlag (2010)
de Carvalho, C. C. C. R., Fernandes, P., "Production of Metabolites as Bacterial Responses to the Marine Environment", Marine Drugs, 8, 705-727 (2010)
Fernandes, P., "Enzymes in Sugar Industries", Enzymes in Food Processing: Fundamentals and Potential Applications, Panesar, P., Marwaha, S. S., Chopra, H. K., New Delhi, I.K. International Publishing House Pvt. Ltd. (2010)
Fernandes, P., "Miniaturization in Biocatalysis", International Journal of Molecular Sciences, 11, 858-879 (2010)
Fernandes, P., Cabral, J. M. S., "Applied Biocatalysis: An overview", Industrial Biotechnology- Sustainable growth and economic success, Soetaert, W., Vandamme, E. J., Weinheim, Wiley-VCH Verlag GnbH (2010)
Fernandes, P., Cabral, J. M. S., "Steroid bioconversions", Encyclopedia of Industrial Biotechnology - Bioprocess, Bioseparation, and Cell Technology, Flickinger, M., New York, John Wiley & Sons (2010)
Marques, M. P. C., Cabral, J. M. S., Fernandes, P., “Bioprocess scale-up: quest for the parameters to be used as criterion to move from microreactors to lab-scale”, J. Chem. Technol. Biotechnol., 85, 1184-1198 (2010)
Marques, M. P. C., Cabral, J. M. S., Fernandes, P., “A microwell platform for the scale-up of a multistep bioconversion to bench-scale reactors: Sitosterol side-chain cleavage” Biotechnol. J., 5, 402-412 (2010)
Marques, M. P. C., Carvalho, F., de Carvalho, C. C. C. R., Cabral, J. M. S., Fernandes, P., “Steroid bioconversion: Towards green processes”, Food Bioprod. Process., 88, 12-20, (2010)
Marques, M. P. C., de Carvalho, C. C. C. R., Cabral, J. M. S., Fernandes, P., “Scaling-up of complex whole-cell bioconversions in conventional and non-conventional media”, Biotechnol. Bioeng., 106, 619-626 (2010)
Marques, M. P. C., Fernandes, P., Cabral, J. M. S., Žnidaršič-Plazl, P., Plazl, I., "On the feasibility of in situ steroid biotransformation and product recovery in microchannels", Chem. Eng. J., 160, 708-714 (2010)
Nunes, M. A. P., Vila-Real, H., Fernandes, P. C. B., Ribeiro, M. H. L., “Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique”, Appl. Biochem. Biotechnol., 160, 2129-2147 (2010)
2009
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., “Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing”, Trends Biotecnhol., 27, 240-247 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., de Vries, J., Visser, T. J.,
Aires-Barros, M. R., "Downstream processing of human antibodies integrating an extraction
capture step and cation exchange chromatography", J. Chromatogr. B, 887,50-58 (2009)
Azevedo, A. M., Gomes, A. G., Rosa, P. A. J, Ferreira, I. F., Pisco, A. M. M. O., Aires-Barros, M. R., “Partitioning of human antibodies in polyethylene glycol - sodium citrate aqueous two-phase systems”, Sep. Purif. Tecnhol., 65, 14-21 (2009)
Azevedo, A. M., Rosa, P. A. J., Ferreira, I. F., Pisco, A. M. M. O., de Vries, J., Korporaal, R., Visser, T. J., Aires-Barros, M. R., “Affinity-enhanced purification of human antibodies by aqueous two-phase extraction”, Sep. Purif. Tecnhol., 65, 31-39 (2009)
Baecker, W., sommerfeld, S., Mutter, M., Rosa, P.A.A.J., Aires-Barros, M.R.M.S., Azevedo, A.M.N.M.P., "Method for purifying therapeutic proteins", World Patent WO/209/112149 (2009)
de Barros, D. P. C. , Fonseca, L. P., Cabral, J. M. S., Weiss, C. K., Landfester, K., “Synthesis of alkyl esters by cutinase in miniemulsion and organic solvent media”, Biotechnol. J.,4, 674-683 (2009)
de Barros, D. P. C. , Fonseca, L. P., Fernandes, P., Cabral, J. M. S., Mojovic L., “Biosynthesis of ethyl caproate and other short ethyl esters catalyzed by cutinase in organic solvent”, J. Mol. Catal. B.: Enzym., 60, 178-185 (2009)
Bernardino, S. M. S. A., Fernades, P., Fonseca, L. P., "A new biocatalyst: Penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties", Biotechnol. J., 4, 674-683 (2009)
Cardoso, M. A. T., Antunes, S., van Keulen, F., Ferreira, B. S., Geraldes, A., Cabral, J. M. S., Palavra, A. M. F., “Supercritical antisolvent micronization of synthetic all trans-beta-carotene with tetrahydrofuran as solvent and carbon dioxide as antisolvent”, J. Chem. Technol. Biotechnol., 84, 215-222 (2009)
Carvalho, F., Marques, M. P. C., Carvalho C. C. C. R., Cabral, J. M. S., Fernandes, P. “Sitosterol bioconversion with resting cells in liquid polymer based systems”, Bioresource Technol., 100, 4050-4053 (2009)
Cattorini, S., Marques, M. P. C., Carvalho, F., Chheub, V. , Cabral, J. M. S. , Fernandes, P. “Lentikat®-based Biocatalysts: Effective Tools for Inulin Hydrolysis”, Chem. Biochem. Eng. Q., 23, 429–434 (2009)
Fernandes, P., Marques, M.P.C., Carvalho, F., Cabral, J.M.S., "A simple method for biocatalyst immobilization using PVA-based hydrogel particles", J. Chem. Technol. Biotechnol. 84, 561-564 (2009)
Marques, M. P. C., Cabral, J. M. S., Fernandes, P., “High throughput in biotechnology: from shake-flasks to fully instrumented microfermentors”, Recent Pat. Biotechnol., 3, 124-140 (2009)
Marques, M. P. C., Carvalho, F., Magalhães, S., Cabral, J. M. S., Fernandes, P., “Characterization of 24-well microtiter plate reactors for a complex multi-step bioconversion: from sitosterol to androstenedione”, J. Biotechnol., 141, 174-180 (2009)
Marques, M. P. C., Carvalho F., Magalhães S., Cabral J. M. S., Fernandes P., "Screening for
suitable solvents as substrate carriers for the microbial side-chain cleavage of
sitosterol using microtitre plates", Process Biochem., 44, 556-561 (2009)
Paixão, L., Rodrigues, L., Couto, I., Martins, M., Fernandes, P., de Carvalho, C.C.C.R., Monteiro, G.A., Sansonetty, F., Amaral, L., Viveiros, M. “Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli”, J. Biol. Eng., 3, 18 (2009)
Pinotti, L. M., Fonseca, L. P., Prazeres, D. M. F., Rodrigues, D. S., Nuccia, E. R., Giordano, R. L. C., “Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column”, Biochem. Eng. J., 44, 111-118 (2009)
Rosa, P .A. J., Azevedo, A. M., Ferreira, I. F., Sommerfeld, S., Bäcker, W., Aires-Barros, M. R., "Downstream processing of antibodies:
Single-stage versus multi-stage aqueous two-phase extraction", J. Chromatogr. A, 1216, 8741-8749 (2009)
Rosa, P .A. J., Azevedo, A. M., Sommerfeld, S., Mutter, M., Aires-Barros, M. R., Bäcker, W., "Application of aqueous two-phase systems to antibody purification:
A multi-stage approach", J. Biotechnol.,139, 306-313 (2009)
Sousa, I. T., Ruiu, L., Lowe, C. R., Taipa, M. A., “Synthetic affinity ligands as a novel tool to improve protein stability”, J. Mol. Recognit., 22, 83-90 (2009)
2008
Azevedo, A. M., Rosa, P. A. J, Ferreira, I. F., Aires-Barros, M. R., "Integrated process
for the purification of antibodies combining aqueous two-phase extraction, hydrophobic
interaction chromatography and size-exclusion chromatography", J. Chromatogr. A, 1213,
154-161(2008)
Baptista, R. P., Pedersen, S., Cabrita, G. J., Otzen, D. E., Cabral, J. M. S., Melo E. P., “Thermodynamics and mechanism of cutinase stabilization by trehalose”, Biopolymers, 89, 538-547 (2008)
Cardoso, F. A., Germano, J., Ferreira, R., Cardoso, S., Martins, V. C., Freitas, P. P., Piedade, M. S., Sousa, L., “Detection of 130 nm magnetic particles by a portable electronic platform using spin valve and magnetic tunnel junction sensors”, J. Appl. Phys., 103,07A310 (2008)
Claudino, M. J. C., Soares, D., Marques, M. P. C., van Keulen, F., Cabral, J. M. S., Fernandes, P., “Immobilization of mycobacterial cells onto silicone - Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol”, Bioresource Technol., 99, 2304-2311 (2008)
Costa, L., Brissos, V., Lemos, F., Ramôa Ribeiro, F., Cabral, J. M. S., “Comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis”, Bioproc. Biosys. Eng., 31, 323-327 (2008)
Costa, L. F. A., Lemos, F., Ramôa Ribeiro, F., Cabral, J. M. S., “Zeolite screening for the racemization of 1-phenylethanol”, Catal. Today, 133-135, 625-631 (2008)
Ferreira, I. F., Azevedo, A. M., Rosa, P. A. J., Aires-Barros, M. R., “Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems”, J. Chromatogr. A, 1195, 94-100 (2008)
Lienqueo, M. E., Salazar, O., Calado, C. R. C., Fonseca, L. P., Cabral, J. M. S., “Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography ”, Biotechnol. Lett., 30, 1353-1358 (2008)
Marques, M. P. C., Cabral, J. M. S., , Fernandes, P. “Online oxygen monitoring system - non-conventional steroid fermentations in microtiter plates”, Bioforum Europe, 9, 42-43 (2008)
Sampaio, P. N., Fortes, A. M., Cabral, J. M. S., Pais, M. S., Fonseca, L. P., “Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain”, J. Biosci. Bioeng., 105, 305-312, (2008)
Santos, A. M., Fedorov, A., Martinho, J. M. G., Baptista, R. P., Taipa, M. A., Cabral, J. M. S., “Orientation of cutinase adsorbed onto PMMA nanoparticles probed by tryptophan fluorescence”, J. Phys. Chem. B, 112, 3581-3585 (2008)
Taipa, M. A., “Immunoassays: Biological tools for High Throughput Screening and characterisation of combinatorial libraries”, CCHTS, 11, 325-335 (2008)
Tavares-Cardoso, M. A., Monteiro, G. A., Cardoso, J. P., Prazeres, T. J. V., Figueiredo, J. M. F., Martinho, J. M. G., Cabral, J. M. S., Palavra, A. M. F., “Supercritical antisolvent micronization of minocycline hydrochloride”, J. Supercritical Fluids, 44, 238-244 (2008)
Vale, G., Mota, A., Fonseca, L., Capelo, J. L., “Ultrasonic assisted enzymatic digestion -USAED- for total elemental determination and elemental speciation: a tutorial”, Talanta, 75, 872-884 (2008)
Vale, G., Pereira, S., Mota, A., Fonseca, L., Capelo, J. L., “Enzymatic probe sonication as a tool for solid-liquid extraction for total selenium determination by electrothermal-atomic absorption spectrometry”, Talanta, 74,198-205 (2008)
Vojinović, V., Cabral, J. M. S., Fonseca, L. P., “Ex-situ bioprocess monitoring techniques”, Chemical Industry & Chemical Engineering Quarterly, 13, 1-15, (2008)
2007
Azevedo, A. M. Rosa, P. A. J., Ferreira, I. F., Aires-Barros, M. R., “Optimisation of aqueous two-phase extraction of human antibodies”, J. Biotechnol., 132, 209-217 (2007)
Carvalho, A. S. L., Ferreira, B. S., Neves-Petersen, M. T., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., “Thermal denaturation of HRPA2: pH-dependent conformational changes”, Enzyme Microb. Technol., 40, 696-703 (2007)
de Carvalho, C. C. C. R., Marques, M. P. C., Fernandes, P., da Fonseca, M. M. R., “Degradation of hydrocarbons and alcohols by Rhodococcus erythropolis DCL14: a comparison in scale performance”, Biocat. Biotrans., 25, 144-150 (2007)
Carvalho, R. H., Lemos, F., Cabral, J. M. S., Ramôa Ribeiro, F., “Influence of the presence of NaY zeolite on the activity of horseradish peroxidase in the oxidation of phenol”, J. Mol. Catal. B., Enzymatic, 44, 39-47 (2007)
Carvalho, R. H., Lemos, F., Lemos, M. A. N. D. A. Cabral, J. M. S., Ramôa Ribeiro, F., “Electro-oxidation of phenol on a new type of zeolite/graphite biocomposite electrode with horseradish peroxidase”, J. Mol. Catal. A, 278, 47-52 (2007)
Catana, R., Eloy, M., Rocha, J. R., Ferreira, B. S., Cabral, J. M. S., Fernandes, P., “Stability evaluation of an immobilized enzyme system for inulin hydrolysis”, Food Chem., 101, 260-266 (2007)
Chen, Z., Franco, C. F., Baptista, R. P., Cabral, J. M. S., Coelho, A. V., Rodrigues Jr, C. J. Melo, E. P., “Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides”, Appl. Microbiol. Biotechnol., 73, 1306-1313 (2007)
Costa, M. J. L., Pedro, L., de Matos, A. P. A., Aires-Barros, M. R., Belo, J. A., Goncalves, J., Ferreira, G. N. M., “Molecular construction of bionanoparticles: chimaeric SIV p17-HIV 1 p6 nanoparticles with minimal viral protein content”, Biotechnol. Appl. Biochem., 48, 35-43 (2007)
Dias, A. I., Feliciano, A. S., Cabral, J. M. S., Prazeres, D. M. F., “Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles”, J. Colloid Int. Sci., 305, 198-201 (2007)
Fernandes, P., Cabral, J. M. S., “Phytosterols: applications and recovery methods”, Bioresource Technol., 98, 2325-2350 (2007)
Lienqueo, M. E., Salazar, O., Henriquez, K., Calado, C. R. C., Fonseca, L. P., Cabral, J. M. S., “Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography”, J. Chromatogr. A, 1154, 460–463 (2007)
Marques, M. P. C., de Carvalho, C. C. C. R., Claudino, M. J. C., Cabral, J. M. S., Fernandes, P., “On the feasibility of the microscale approach for a multi-step biotransformation: sitosterol side-chain cleavage”, J. Chem. Technol. Biotechnol., 82, 856-863 (2007)
Madeira, C., Loura, L., Prieto, M., Fedorov, A., Aires-Barros, M. R., “Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size”, Eur. Biophys. J. Biophys., 36, 609-620 (2007)
Neves-Petersen, M. T., Klitgaard, S., Carvalho, A. S. L., Petersen, S. B., Aires-Barros, M. R., Melo, E. P., “Photophysics and photochemistry of horseradish peroxidase A2 upon UV illumination”, Biophys. J., 92, 2016-2017 (2007)
Otzen, D. E., Giehm, L., Baptista, R. P., Kristensen, S. R., Melo, E. P. Pedersen, S., “Aggregation as the basis for complex behaviour of cutinase in different denaturants”, Biochim. Biophys. Acta, 1774, 323-333 (2007)
Rosa, P. A. J., Azevedo, A. M., Aires-Barros, M. R., “Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies”, J. Chromatogr. A, 1141, 50-60 (2007)
Rosa, P. A. J., Azevedo, A. M., Ferreira, I. F., de Vries, J., Korporaal, R., Verhoef, H. J., Visser, T. J., Aires-Barros, M. R., “Affinity partitioning of human antibodies in aqueous two-phase systems”, J. Chromatogr. A, 1162, 103-113 (2007)
Roque, A. C. A., Silva, C. S. O., Taipa, M. A., “Affinity-based methodologies and ligands for antibody purification: Advances and Perspectives”, J. Chromatogr. A, 1160, 44-55 (2007)
Vojinovic, V., Carvalho, R., Lemos, F., Cabral, J. M. S., Fonseca, L. P., Ferreira, B. S., “Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds”, Biochem. Eng. J., 35, 126-135 (2007)
